ISSN 1995-5537
«Біотехнологія» journal V. 5, No. 5, 2012
Р. 100-108, Bibliography 20, Ukrainian.
Universal Decimal classification: 577.1:616-003.269:636
M. Ye. Roman’ko, L. S. Rieznichenko
National Scientific Center «Institute of Experimental and Clinical Veterinary Medicine», Kharkiv
Ovcharenko Institute of Biocolloidal Chemistry
of National Academy of Sciences of Ukraine, Kyiv
The effect of argentum and aurum nanoparticles and proliferation parameters, respiratory activity and permeability of cell membranes, the activity of the membrane H+-ATPase and catalase, total antioxidant activity of the bacterial cell of Escherichia coli and Pasteurella multocida after lyophilization and long-term storage were studied. It was determined that as a result of processing aurum nanoparticles at a concentration of 1.93 mg / ml in metal and argentum — 8,64 mg / ml bacterial cells Escherichia and Pasteurella before freeze-drying step stimulation of biomass growth, modulation of enzyme activity of cellular energetic, the stabilization of cell membranes and activation of the antioxidant activity of the cells were observed. This is an evidence of marked activation of repair processes of bacterial cells during their rehydration under the influence of metal nanoparticles studied. These features influence the investigated nanoparticles aurum and argentum on cells of Escherichia and Pasteurella after lyophilization/rehydration, afford grounds for useing of nanoparticles as a crioprotectors and/or modulators in the technology of long-term storage of industrial strains of microorganisms.
Key words: industrial strains of microorganisms, nanoparticles, aurum, argentum, contact interaction, lyophilization/rehydration, longterm storage.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008