ISSN 1995-5537
«Біотехнологія» journal V. -, No. 4, 2012
Р. 88-95, Bibliography 26, Ukrainian.
Universal Decimal classification: 759.873.088.5:661.185
T. P. Pirog, T. A. Shevchuk, M. A. Shulyakova
Zabobltny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv
National University of Food Technologies, Kyiv
The possibility of intensification of biosurfactant synthesis by strain Acinetobacter calcoaceticus IMV В-7241 on glycerol in the presence of fumarate (C4-dicarboxylic acid, precursor of gluconeogenesis) and citrate (lipid synthesis regulator) was found.
Complex addition into the medium with glycerol (1%, v/v) of fumarate and citrate (0,01–0,02%) was accompanied by increasing of the exocellular biosurfactant quantity in 2–2,5 times compared to cultivation of IMV В-7241 strain on medium without organic acids.
Increase in surfactant concentration was arisen from simultaneous functioning of two anaplerotic ways (both the glyoxylate cycle and phosphoenolpyruvate(PEP)-carboxylase reaction) as well as enhancing in 3–5 times of enzyme activity of biosynthesis of surface-active glyco-(phosphoenolpyruvate synthetase and phosphoenolpyruvate carboxykinase) and aminolipids (NADP+-dependent glutamate dehydrogenase).
Maximal characteristics of A. calcoaceticus ІМV В-7241 biosurfactant synthesis on glycerol were observed in the presence of yeast autolisate and microelements in the medium if inoculums was used that had been grown in medium without growth factors.
In the presence of ferrous sulfate in glycerol containing medium for inoculums obtaining and bacteria culturing, synthesis parameters of surfactants decreased due to Fe2+ inhibitory effect on activity of enzyme biosynthesis of IMV B-7241 strain.
The obtained data are bases for development of new technology of biosurfactant synthesis by strain A. calcoaceticus ІМV В-7241.
Key words: biosurfactants, intensification of synthesis, organic acids, glycerol, Acinetobacter calcoaceticus IMV В-7241.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008