ISSN 1995-5537
«Біотехнологія» journal V. 5, No. 4, 2012
Р. 96-103, Bibliography 24, Ukrainian.
Universal Decimal classification: 612.419-014.3:612.75
State Institute of Genetic and Regenerative Medicine of National Academy of Medicine of Ukraine, Kyiv
Multipotent mesenchymal stromal cells from bone marrow of FVB mice for colonies forming activity were studied. The percentage of the colony forming units for every sample was measured. Also there were assessed levels of expression of the surface markers common for multipotent stromal cells: CD34, CD44, CD45, CD73, CD90, CD117. According to expression of these markers, the cells correspond to characteristics of adult multipotent mesenchymal stromal cells from bone marrow. Components for chondrogenic differentiation were analyzed and the most essential were used in our researches. It was shown that multipotent mesenchymal stromal cells could be differentiated into chondrogenic direction in vitro as a monolayer. The combined dying technique was applied to the fixed monolayer of differentiated cells. The sequential use of alcian blue and safranin O demonstrated the distribution of glycosaminoglycans and proteoglycans which are typical for chondrogenic tissue in the cells and in the extracellular matrix.
Key words: cell culture, multipotent stromal stem cells, chondrogenesis, directed differentiation.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008