ISSN 1995-5537

«Біотехнологія» journal V. 5, No. 4, 2012
Р. 104-111, Bibliography 15, Ukrainian.
Universal Decimal classification: 535.373

PROTEINS AND POLYRIBONUCLEOTIDES LUMINESCENCE.
THE POSSIBILITY OF SPECTRAL TESTING OF THEIR INTERACTION

V. M. Yashchuk, Z. Yu. Tkachuk, S. M. Levchenko, V. Yu. Kudrya, V. I. Mel’nik, V. P. Vorob’yov

Taras Shevchenko National University of Kyiv
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Institute of Physics of National Academy of Sciences of Ukraine, Kyiv

Possibilities of application of a low-temperature luminescence method for the detection of binding between proteins and nucleic acids, oligonucleotides and other compounds were studied. The position of the first excited singlet and triplet electronic energy levels of the ribonucleotides and π-electron containing amino acids were evaluated using absorption and luminescence spectra. It was confirmed that the phosphorescence spectra of RNA were connected with the adenine base emission that possessed the lowest triplet level in the RNA bases triplet levels systems. It was shown that the low-temperature autophosphorescence of proteins and nucleotides (due to the sharp spectra’s structure) gave the possibility to determine presence of binding between components in complex biological system. The spectral evidences of the binding between human albumin molecules and 2^'-5^'oligoadenilate molecules were given.

Key words: RNA, human albumin, amino acids, nucleotides, π-electron contained systems, 2^'-5^'olygoadenilate, luminescence, absorption, energy levels, spectral displays of interaction.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008