ISSN 1995-5537
«Біотехнологія» journal V. 4, No. 4, 2012
Р. 65-73, Bibliography 47, Ukrainian.
Universal Decimal classification: 631.811.9875.117.2.577.2.08
V. А. Tsygankova, S. P. Ponomarenko, A. P. Galkin, A. I. Yemets
Institute of Bioorganic Chemistry and Petrochemistry National Academy of Sciences of Ukraine, Kyiv
National Enterprise Interdepartmental Science & Technology Center «Agrobiotech»
of National Academy of Sciences and Ministry of Education and Science of Ukraine, Kyiv
Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine, Kyiv
Root culture of four lines chicory (Cichorium intybus L.), transformed by Аgrobacterium rhizogenes, showed the considerable increase of polyfructan (PF) synthesis level in nutrient media with growth regulator Charkor. It is shown that adding growth regulator Charkor into medium with agar and liquid medium 1/2МС increased root mass (by dry mass) in 39–54 times relative to control depending on concentration of regulator (from 2,5 to 10 ?l/l of medium). Influence of growth regulator Charkor on growth of root biomass was similar to action of auxin, in particular, indolebutyric acid. Adding of growth regulator Charkor to these media diminished the relative concentration of polyfructans (mg/g of dry mass) in 2–8 times, that is related to hastier root growth on such conditions. At the same time when cultivation of root is done at presence of growth regulator Charkor, the general maintenance of polyfructans (48–130 mg/g of total dry root mass grown for a 30 twenty-four hours) was well above than in the control.
The method of DOT-blot hybridization showed a considerable difference in populations of cytoplasmic mRNA and small regulatory si/miRNK between control and experimental plants. It is indicative of some changes in gene expression under the influence of the regulator.
Key words: plant growth regulator Charkor, chicory «hairy roots», polyfructans, DOT-blot-hybridization.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008