ISSN 1995-5537
«Біотехнологія» journal V. 5, No. 4, 2012
Р. 55-64, Bibliography 45, Ukrainian.
Universal Decimal classification: 577.29:577.27
ALLOGENEIC SCREENING OF TUMOR-ASSOCIATED ANTIGENS
OF HUMAN BREAST CANCER
O. I. Kostianets, M. A. Shyian, S. V. Antoniuk, S. V. Demidov, V. V. Filonenko, R. G. Kiyamova
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Taras Shevchenko National University of Kyiv
Dnipropetrovs’k Municipal Clinical Hospital Nо. 4
Breast cancer is the most common type of malignancy among women. Despite the using of mammography screening programs, diagnostics of this pathology is still not effective enough, so there is a need to search for new diagnostic markers of breast cancer. Tumor-associated antigens and their cognate autoantibodies, which have several advantages compared to other biological molecules can be considered as cancer biomarkers and used for cancer diagnostics and prognosis.
In current study we characterized in allogeneic screening recombinant analogues of tumor-ssociated SEREX (Serological identification of antigens by recombinant expression cloning)-antigens with sera from patients with different types of breast cancer and sera of healthy donors using enzyme-linked immunoenzyme assay. We identified combination of antigens, which could be used for further research in context of test-systems development for autoantibodies profile identification for breast cancer diagnostics.
Key words: tumor-associated antigen, autoantibody, breast cancer.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008