ISSN 1995-5537
"Biotechnology" journal V. 5, No. 3, 2012
Р. 98-104, Bibliography 21, Russian.
Universal Decimal classification: 579.254.2:581.143.5
S. I. Mykhalska, N. I. Adamenko, B. V. Morgun, A. V. Kochetov, O. M. Tishchenko
Institute of Plant Physiology and Genetics of National Academy of Science of Ukraine, Kyiv
Institute of Cell Biology and Genetic Engineering of National Academy of Science of Ukraine, Kyiv
Institute of Cytology and Genetics of Russian Academy of Science, Novosibirsk
Agrobacterium-mediated transformation is an important experimental tool for the development of molecular biotechnologies. But the genetic improvement of majority commercial maize lines is limited by the lack of effective method system of regeneration and transformation. The possibility of using of segments (1-2 mm) of cross section nodal area of 7-10 days old shoots as explant for Agrobacterium-mediated transformation of elite corn inbred line L250, L390, L1544, L1552, L1563, L1652 using strain of LВА4404 harboring binary vector pBi2E with ds-RNA suppressor for proline dehydrogenase with selective gene neomycinphosphotransferase II was analyzed. Regeneration of shoots transformed by through direct organogenesis was established independent from genotype after 10–14 days and shortly afterwards rooting induction were observed. It has been shown that introduction into cells and corn genome integration of T-DNA may be take place with sodium thiosulfate without acetosyringone. The availability of using of cross section nodal as explant for develop the method of Agrobacterium-mediated transformation of elite corn inbred line are shown.
Key words: Zea mays L., Agrobacterium-mediated transformation, direct organogenesis, cross section nodal area, thiosulfate Na.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008