EFFECTS OF ALLOPLASMIC INTERACTIONS IN THE HIGHER PLANT RECIPROCAL CYBRIDS Y. I. Ratushnyak, A. S. Kochevenko

Details: Hits: 344

ISSN 1995-5537

"Biotechnology" journal V. 5, No. 1, 2012
Р. 18-32, Bibliography 66, Russian.
Universal Decimal classification: 575.127

EFFECTS OF ALLOPLASMIC INTERACTIONS IN THE HIGHER PLANT RECIPROCAL CYBRIDS

Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv

Methods of reciprocal cytoplasmic hybrids obtaining between phylogenetically remote taxa of flowering plants and different effects of the novel nucleocytoplasmic interactions are dicussed in the review. Cybrid selection system aimed towards chloroplasts transfer from donor was applied in all cases of protoplast fusion. In terms of phenotypic and photosynthetic charac ters due to disturbances of alloplasmic interac tions in the direct and reverse cybrids of tobacco or cultivated tomato, essential reciprocal differences have been registered. Principal advantages and key achievements of alloplasmic lines and cytoplasmic hybrids of the higher plants which are suggested as model objects in molecular biological, genetic, physiological and biotechnologi cal studies are also discussed in the review.

Key words: Nicotiana, Atropa, Lycopersicon, reciprocal cytoplasmic hybrids, phenotypic and photosynthetic effects, nucleocytoplasmic incompatibility.