ISSN 1995-5537

"Biotechnology" journal V. 4, No. 6, 2011
Р. 36-41, Bibliography 12, Russian.
Universal Decimal classification: 577.152.3.04

USE OF CHEMICAL MODIFICATION METHODS FOR STABILIZATION OF FUNGAL GLYCOSIDASES

N. V. Borzova, L. D. Varbanets

Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv

Urgent task of the modern applied enzymology, in particular enzyme engineering technique, is increasing of enzyme stability in different, often extreme, conditions of the reaction medium. Comparative study of thermostability of α-galactosidases native and modified by various methods out of three fungal producers — Aspergillus niger, Penicillium canescens and Cladosporium cladosporioides indicated that treatment with cellulose, dextrans Т500, Т20 and also 6 000 and 1 500 PEG stabilized the enzymes at 55 ^oС and 60 ^oС. An approach based on hydrophobic modification of enzymes by acylation of aminogroups of lysine and glycine with 2,6-diaminoipimelic acid and glycine appeared to be effective. As a result of such interaction thermostability of α-galactosidase was increased in 1,5–5 times.

Key words: α-galactosidases Aspergillus niger, Penicillium canescens, Cladosporium cladosporioides, thermal stabilization, chemical modification of enzymes.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008