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ISSN 1995-5537

 

 5 2011

"Biotechnology" journal V. 4, No. 5, 2011
Р. 25-38, Bibliography 31, Ukrainian.
Universal Decimal classification: 575.23+615.222:581.143.6

THE DYNAMICS OF CELL SYSTEMS in vitro.
І. TEMPORAL ORGANIZATION AND STABILITY
OF Rauwolfia serpentina CULTURE TISSUES AT CIRCADIAN LEVEL

N. Yu. Мiryuta, V. А. Kunakh

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv

Growth and development as a complete organism as a plant tissue culture were determined by oscillation changes of the cell system dynamics characteristics that formed corresponding tissues or an organism as a whole. However these basic processes are not well understood.

The dynamics of cell systems has been studied on the example of Rauwolfia serpentine Benth culture tissues, in particular their organization in time for the daily level of the hierarchy. The phase trajectories of the cell part dynamics with different DNA content in the nucleus and different nucleolus area, as well as dynamics of proliferation indexes (mitosis and amitosis) under variation of cultivation conditions for the high-productive Rauwolfia serpentina K-27 strain (antiarhythmic alkaloid ajmaline producer) were determined by Liapunov indexes. The applied biotechnological methods of productivity increasing in the researched cell culture have been assumed to use dynamics hereditary memory mechanisms.

Key words: cell population in vitro, dynamic of the cell systems, phase trajectories, Rauwolfia serpentina.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008