ISSN 1995-5537
"Biotechnology" journal V. 4, No. 5, 2011
Р. 39-44, Bibliography 14, Ukrainian.
Universal Decimal classification: 579.841:577.15
O. V. Karpenko, M. V. Prystay, G. V. Gafijchuk, B. Y. Datsko, T. M. Nogyna
Department of Physical Chemistry of Combustible minerals of Lytvynenko Institute of Physic, Organic and Coal Chemistry of National Academy of Sciences of Ukraine, Kyiv;
Pidstryhach Institute of Applied Problems of Mathematics and Mechanics
of National Academy of Sciences of Ukraine, Kyiv;
Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv
By a regressive and dispersion analysis, the optimum concentrations of carbon and nitrogen sources were determined in nutrient solution composition for obtaining of biomass, cell lipids and exopolysaccharide of Gordonia rubripertincta UKM Ace-122 strain.
The nutrient solution composition was optimized for Gordonia rubripertincta UKM Ace-122 strain cultivation as follows: the concentrations of carbon and nitrogen sources were selected for maximum yield of biomass, biosurfactant and exopolysaccharides. Regression dependences were analyzed for each biosynthesis product, enabling to increase biomass accumulation in 1.3 times, the cell lipid content in 1.9 times (from 4.35 to 8.12 g/l) and exopolysaccharides — in 5.1 times (from 1.54 to 7.89 g/l).
The main trends of influence of the used environment components on the studied biotechnological indexes were determined. These results were confirmed experimentally.
Key words: biosurfactant, exopolysaccharides, media optimization, Gordonia, regression polynomial, regression analysis.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008