ISSN 1995-5537
"Biotechnology" journal V. 4, No. 5, 2011
Р. 57-63, Bibliography 25, Ukrainian.
Universal Decimal classification: 576.314+576.367+571.27
MEMBRANE VESICLES FORMATION TECHNOLOGY
FOR ANTICANCER IMMUNOTHERAPY
Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv
The article is devoted to discussion of the mechanisms of inducible formation of cancer cells membrane vesicles, which would maintain their immunological (antigenic) properties and would become a perspective agent for anticancer immunotherapy. Anticancer immunotherapy is able to provide immune response of the organism towards cancer cells, and prevent the development of micro-metastases and to facilitate the elimination of the remnants of these cells, that remain after other treatments. To provide an immune response towards tumor antigens one should carry out an organism immunization with the appropriate tumor surface antigens. Some methods of immunization with inactivated or apoptotic cancer cells are known. The last provoke exposure phosphatidyl serine on their surface, which, due to its anti-inflamatory properties, making it impossible to use them for immune response induction. The work objective was screening of the agents, able to induce formation of membrane vesicles that would maintain antigenic properties of tumor and contain little of phosphatidyl serine. We have shown that inhibitor of ATP-sensitive potassium channels of endoplasmic reticulum, glibenclamide, induces formation of membrane vesicles with «non-apoptotic» phenotype, characterized by relatively small (< 2,5 μm) in size, low phosphatidyl serine content, but at the same time expressing glycocalyx composition on its surface. The combined effect of glibenclamide and ultraviolet radiation type B (315–280 nm) significantly increased the amount of formed membrane vesicles with low-phosphatidyl serine content but maintaining glycocalyx components (as main immunological determinants). These vesicles and their creation technology could be a promising mean to carry out immunization uring autologous immunotherapy of tumors.
Key words: plasmatic membrane, membrane blebs, anticancer immunotherapy, phosphatidyl serine, apoptosis.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008