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ISSN 1995-5537

"Biotechnology" journal V. 4, No. 3, 2011
Р. 51-55, Bibliography 9, Russian.
Universal Decimal Classification: 579.222

Influence of enzymatic medium components on tryptophan biosynthesis

Shulga S. M., Tkachenko A. F., Tigunova O. O., Beyko N. E. Khomenko A. I.

State organization «Institute of Food Biotechnology and Genomics»
of National Academy of Sciences of Ukraine, Kyiv

Bacterial cultures out of «Collection microorganism’s stains and plants line for food and agriculture biotechnology» of the Institute of Food Biotechnology and Genomics of NAS of Ukraine have been studied. The tryptophan producer stains Corynebacterium glutamicum Т-3, which produced 4 g/dm3 of tryptophan in cultural liquid were studied. Influence of some carbon source on tryptophan biosynthesis was observed. The most activity of tryptophan biosynthesis with molasses was shown. It was considered some tryptophan precursors in biosynthesis: indole, indolepyruvic acid, indolelactic acid; the following mixtures: indole + L-serine, indole + L-cysteine, indole + DL-alanine. Application of tryptophan synthesis precursors to medium stimulates tryptophan accumulation in cultural liquid. Possibility to receive economically available tryptophan was shown provided that these expensive precursors were exchanged for cheaper source, namely molasses.

Key words: tryptophan, Corynebacterium glutamicum Т-3, biosynthesis, amino acids.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008