ISSN 1995-5537
"Biotechnology" journal V. 4, No. 2, 2011
Р. 73-79, Bibliography 10, Ukrainian.
Universal Decimal classification: 57.083.2./.3:575:582.926.2:619:616.98
PRODUCTION OF BLUETONGUE VP7 VIRUS ANTIGEN IN Nicotiana tabacum TRANSGENIC PLANTS
Ukrainian Laboratory of Quality and Safety of AIC Products,
National University of Life and Environmental Science of Ukraine, Kiyv
The method of receiving immunologically active and group specific antigen Vp7 of ruminant bluetongue virus in the transgenic Nicotiana tabacum plants is developed. The lines of transgenic tobacco, homozygous for kanamycin resistance trait have been done with the maximal level of recombinant antigen VP7 expression. It was shown that the total yield of the purified antigen composed 0.2 mg per 1 gram of plant biomass. The use of recombinant antigen VP7 in veterinary practice gives a possibility to develop ELISA kits for the serological monitoring and to prevent bluetongue distribution on territory of Ukraine.
Key words: bluetongue of ruminant, transgenic plants, recombinant antigens, ELISA.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008