ISSN 1995-5537
"Biotechnology" journal V. 4, No. 2, 2011
Р. 80-85, Bibliography 17, Ukrainian.
Universal Decimal classification: 579.22: 579.243.6: 631.461.61
SELECTION OF ANAEROBIC CELLULOLYTIC THERMOPHILIC
STRAIN Сlostrіdіum thermocellum 5СT
L. S. Yastremskaya, O. A. Vasylchenko
National Aviation University, Institute for Environmental Security, Kyiv
Selection of anaerobic thermophilic cellulolytic strain of Clostridium thermocellum 5ST was performed under multiple re-inoculations and effect of increasing concentrations of the exogenous compounds. Culture growth was evaluated by biomass formation, final metabolite products accumulation, and intensity of cellulose hydrolysis. Obtained Clostridium thermocellum 5СТ culture was resistant to ethanol at concentration 2%. It is shown that hydrogen adding to culture medium up to 100% vol. doesn’t inhibit growth of the culture, results in increasing of ethanol formation in two times, and decreases acetate formation in 2.5 times. Sodium acetate adding in low concentrations doesn’t inhibit growth of the culture, whereas 50.0% vol. in the media inhibits essentially growth of the culture; cellulose hydrolysis in this case decreases in 1.5 times.
Selected anaerobic cellulolytic thermophilic strain C. thermocellum 5СТ can be used in biotechnological processes for perspective energy carriers and thermostable enzymes obtaining.
Key words: anaerobic cellulolytic thermophilic bacteria, Clostrіdіum thermocellum, cellulose fermentation.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008