ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 6, 2010
Р. 80-84, Bibliography 7, Ukrainina.
Universal Decimal classification: 621.59:597-114.78
REPRODUCIBILITY OF THE RESULTS OF OOCYTES IMPREGNATIOBY
DECONSERVED CARP SPERM (Cyprinus carpio L.)
L. V. Gorbynov, V. Y. Filipov, L. P. Buchatsky
Institute of Stock-Raising of Ukrainian Academy of Agricultural Sciences, Kharkiv;
Institute of Fish Economy, Kyiv
For estimation of the chosen method of cryopreservation of carp reproductive cells, a method of the results reproducibility increasing of oocyte impregnation was offered. It gave a possibility to reduce influence of individual properties of reproductive cells and carp ovules, terms of conducting of impregnation and cultivation more than twice upon estimating the cryopreservation method. It was determined by dispersion analysis that different quality of reproductive cells and oocytes had force of influence η2А = 0,31 on impregnation cage ability, while cryopreservation stages particularly temperature adaptation of reproductive cells, their self-control in cryopreservation, freezing-thawing, impregnation of ovules and cultivation was η2B = 0,61.
Key words: cryoconservation–deconservation of sperms, oocyte, producers, safety of gametes, reproducibility.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008