ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 6, 2010
. 75-79, Bibliography 14, Russian.
niversal Decimal classification: 57.085.23:616.361:616-78

IONIZING IRRADIATION EFFECT ON PORCINE ARTERIES
FOR CREATION OF DEVITALIZED SСAFFOLDS

D. V. Byzov, O. P. Synchikova, I. P. Mikhaylova, B. P. Sandomirskiy

Institute of Problems of Cryobiology and Cryomedicine
of National Academy of Sciences of Ukraine, Kharkiv

An actual task of tissue engineering is the development of adequate devitalization method of xenogenic arteries to create the small diameter vascular prostheses. Detergent-enzyme treatment is accompanied with some quite serious side effects. A new approach to devitalization of xenoarteries using the effect of damage by low temperatures and ionizing radiation is under our development. In the paper the experimental findings on the effect of ionizing radiation on morphological and functional characteristics of porcine arteries are presented. The studied radiation doses have been shown to cause deendothelization of arteries, higher ones also caused interphase death of smooth muscle cells. Structural integrity of connective tissue fibers of vascular wall is not impaired. The selective rise of the strength parameters of irradiated arteries in radial direction has been revealed as well.

Key words: scaffolds, vascular grafts, devitalization, bioengineering, xenogenic arteries.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008