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ISSN 1995-5537

1 2010

"Biotechnology" journal Vol. 3, No. 1, 2010
Р. 52-57, Bibliography 21, Russian.
УДК: 57.085.23:615.361.

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of  Ukraine, Kharkіv
State Research Control Institute of Biotechnology and Microorganisms Strains, Kyiv
The influence of the low-polymeric fraction below 5 kD extracted from cattle cord blood and Actovegin upon adhesive properties of diploid culture of human embryonic fibroblasts and continuous cell line BHK-21 clone 13 was studied. It was shown that adding of the abovementioned preparations to regenerative medium compositions increased efficiency of attachment of cryopreserved continuous line and did not affect the number of attached cells of cryopreserved diploid culture. It also enhanced the rate of cell spreading on substrate and assuming the typical morphology by cell of the cultures investigated on a substrate. A higher efficiency of cord blood fraction compared to Actovegin in regenerative media was demonstrated by indices assessed.
Key words: low-molecular fraction from cord blood, cell cultures, cryopreservation, regenerative media, adhesion

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008