"Biotechnology" journal Vol. 2, No. 3, 2009
Р. 86-93, Bibliography 23, Ukrainian.
Universal Decimal classification 577.151.4+543.555+544.475
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Taras Shevchenko National University of Kyiv
Data of conductometric biosensor optimization work for heavy metal ions determining in the real samples are presented. A differential pair of the planar gold electrodes deposited on the pyroceramic substrate was used as a conductometric transducer. Threeenzymes system (invertase, mutarotase, glucoseoxidase) immobilized on a transducer’s surface was used as a bioselective element. Developed biosensor demonstrated a good reproducibility of signal. Optimal concentration of sucrose for inhibition analysis was 1.25 mM and incubation time in investigated solution amounted to 10–20 min. Developed biosensor demonstrated the best sensitivity towards ions Hg2+ и Ag+. Principal capability of biosensor reactivation by EDTA solution after inhibition by silver ions or cysteine solution after inhibition by mercury ions was shown. Results of biosensor analysis of toxicants in real water samples have a positive correlation with the results obtained by traditional methods.
Key words: biosensor, heavy metals, inhibitory analysis, enzymes, conductometric transducer.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008