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ISSN 1995-5537

1 2008

“Biotechnology” journal Vol. 1, No.1, 2008
P. 80-84, Bibliography 14, Ukrainian.
Universal Decimal classification: 578.245:577.1113.7:57.086.833

ESTIMATION OF EFFECTIVENESS OF EXPERIMENTAL DEVICE FOR OBTAINING OF TYPE I INTERFERONS

Yu. M. Penchuk, O. V. Karpov V. M. Povodzinsky S. V. Veriovka, Z. R. Ul’berg

National Universyti of Food Technologies, Kyiv
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
Kolomiychenko Institute of Otolaringology of Academy of Medical Sciences of Ukraine, Kyiv
Ovcharenko Institute of Biocolloid Chemisrty
Ovcharenko Institute of Biocolloid Chemisrty of the Nationale Academy of Sciences of Ukraine, Kyiv

The authors describe an experimental device with computer assisted action for cell cultivation and induced type I interferon (IFN) synthesis. The device is based on the use of previously elaborated particles bearing an immobilized complex inductor of repeated action.

The device is shown to be effective for cultivation of both monolayer and suspended cells producing the IFN. Physiological conditions of both cell types cultivation are investigated. The authors conclude their device to be useful for obtaining of biologically active substances by this technology.

Key words: interferon, inductor, immobilization, cpheron, cell culture, roller cultivation, mononuclears.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008