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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 11, No 1, 2018
Р. 5-24, Bibliography 121, English
Universal Decimal Classification: 579.64:581.1
https://doi.org/10.15407/biotech11.01.005
MICROBIAL SYNTHESIS OF PHYTOHORMONES
T. P. Pirog 1, 2, G. O. Iutynska 1, N. O. Leonova 1, K. A. Beregova 2, T. A. Shevchuk 1
1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2 National University of Food Technologies, Kyiv, Ukraine
The aim of the review was to analyze current literature data and the results of own studies on the synthesis of auxins, cytokinins, and gibberellins by plant-associated microorganisms (living in rhizosphere, endophytic, nitrogen-fixing, and phytopathogenic), and by those not involved in symbiotic interactions. Many microorganisms can generate phytohormones, and microbial synthesis of indole-3-acetic acid can be enhanced which can be used in producing it instead of extracting it from plants or by chemical synthesis. Recent progress in intensifying the synthesis of gibberellic acid in deep and solid-phase producer cultivation allows substantially reducing the prime cost of biotechnological production of that phytohormone. The ability of microorganisms to simultaneously synthesize phytohormones and other biologically active compounds with antimicrobial, nematocidal, and other various effects enables creating complex polyfunctional microbial preparations with various biological properties for use in crop production to stimulate plant growth and pest control.
Key words: phytohormones, microbial synthesis, complex microbial preparations
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
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99. Maruyama A., Maeda M., Simidu U. Occurrence of plant hormone (cytokinin) — producing bacteria in the sea. J. Аppl. Microbiol. 1986, 61 (6), 569–574.
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103. Khan A. L., Hussain J., Al-Harrasi A., Al-Rawahi A., Lee I. J. Endophytic fungi: resource for gibberellins and crop abiotic stress resistance. Crit. Rev. Biotechnol. 2015, 35 (1), 62–74. doi: https://doi.org/10.3109/07388551.2013.800018
104. Leit?o A. L., Enguita F. J. Gibberellins in Penicillium strains: Challenges for endophyte-plant host interactions under salinity stress. Microbiol. Res. 2016, V. 183, P. 8–18. https://doi.org/10.1016/j.micres.2015.11.004
105. Jaroszuk-?cise? J., Kurek E., Trytek M. Efficiency of indoleacetic acid, gibberellic acid and ethylene synthesized in vitro by Fusarium culmorum strains with different effects on cereal growth. Biologia. 2014, 69 (3), 281–292.
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107. Meleigy S. A., Khalaf M. A. Biosynthesis of gibberellic acid from milk permeate in repeated batch operation by a mutant Fusarium moniliforme cells immobilized on loofa sponge. Bioresour. Technol. 2009, 100 (1), 374–379. doi: 10.1016/j. biortech.2008.06.024.
108. Lale G., Gadre R. Enhanced production of gibberellin A4 (GA4) by a mutant of Gibberella fujikuroi in wheat gluten medium. Ind. Microbiol. Biotechnol. 2010, 37 (3), 297–306.
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110. Muromtsev G. S., Krasnopolskaya L. M. Micromycetes strain Fusarium monili forme — producer of phytohormons gibberellins A4, A7. RF Patent № 2084531. Publ. 20.07.1997. (In Russian).
111. Eleazar M. E. S., Dendooven L., Maga?a I. P., Parra R., De laTorre M. Optimization of gibberellic acid production by immobilized Gibberella fujikuroi, mycelium in fluidized bioreactors. J. Biotechnol. 2000, 76 (2–3), 147–155.
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113. Machado C. M. M., Soccol C. R., Pandey A. Gibberellic acid production by solid state fermentation in coffee husk. Appl. Biochem. Biotechnol. 2002, V. 102, P. 179–192.
114. Corona A., Sanchez D., Agostin E. Effect of water activity on gibberellic acid production by Gibberella fujikuroi under solid-state fermentation conditions. Proc. Biochem. 2005, V. 40, P. 2655–2658.
115. de Oliveira J., Rodrigues C., Vandenberghe L. P. S., C?mara M. C., Libardi N., Soccol C. R. Gibberellic acid production by different fermentation systems using citric pulp as substrate/support. Biomed. Res. Int. 2017; 2017:5191046. https://doi.org/10.1155/2017/5191046
116. Hori K., Ichinohe R., Unno H., Marsudi S. Simultaneous syntheses of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa IFO3924 at various temperatures and from various fatty acids. Biochem. Eng. J. 2011, 53 (2), 196–202.
117. Liang T. W., Wu C. C., Cheng W. T., Chen Y. C., Wang C. L., Wang I. L., Wang S. L. Exopolysaccharides and antimicrobial biosurfactants produced by Paenibacillus macerans TKU029. Appl. Biochem. Biotechnol. 2014, 172 (2), 933–950.
118. Sharma D., Singh Saharan B. Simultaneous production of biosurfactants and bacteriocins by probiotic Lactobacillus casei MRTL3. Int. J. Microbiol. 2014, 2014:698713. https://doi.org/10.1155/2014/698713
119. Pirog T. P., Konon A. D., Sofilkanich A. P., Iutinskaya G. A. Effect of surface-active substances of Acinetobacter calcoaceticus IMV B-7241, Rhodococcus erythropolis IMV Ac-5017, and Nocardia vaccinii K-8 on phytopathogenic bacteria. Appl. Biochem. Microbiol. 2013, 49 (4), 3604–367. https://doi.org/ 10.1134/S000368381304011X.
120. Pirog T. P., Leonova N. O., Shevchuk T. A., Panasuk E. V., Beregovaya K. A., Iutynskaya G. A. Synthesis of phytohormones by Nocardia vaccinii IMV B-7405 — producer of surfactants. Mikrobiol. Zh. 2015, 77 (6), 21–30. (In Russian).
121. Pacwa-P?ociniczak M., P?ociniczak T., Iwan J., ?arska M., Chor??ewski M., Dzida M., Piotrowska-Seget Z. Isolation of hydrocarbon-degrading and biosurfactantproducing bacteria and assessment their plant growth-promoting traits. J. Environ. Manage. 2016, V. 168, P. 175–84. https://doi.org/10.1016/j.jenvman.2015.11.058
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"Biotechnologia Acta" V. 11, No 1, 2018
Р. 76-81, Bibliography 25, English
Universal Decimal Classification: 576.3+612.014.2/3
https://doi.org/10.15407/biotech11.01.076
ISOLATION OF MULTIPOTENT MESENCHIMAL STROMAL CELLS FROM MINIMAL HUMAN ENDOMETRIUM BIOPSY
1State Institute of Genetic and Regenerative Medicine of National Academy of Medical Sciences of Ukraine, Kiev
2Biotechnology Laboratory “ilaya Regeneration”, Medical Company ilaya, Kiev
3Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
The aim of the research was establishing a cell culture from a minimal human endometrial biopsy and assessment its conformity with the criteria for multipotent mesenchymal stromal cells. It was shown that cells in the culture possess adhesion to plastic, have characteristic fibroblast-like morphology, express CD73+CD90+CD105+, and are negative for hematopoietic markers (CD34-CD45-HLA-DR-), have the ability to directed adipogenic, osteogenic and chondrogenic differentiation. Due to these properties, the cell population isolated from the minimal endometrial biopsy can be attributed to multipotent mesenchymal stromal cells.
Key words: human endometrium, multipotent mesenchymal stromal cells.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
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thin endometrium in ART programs. Problemy reproduktsiyi.2013, V. 4, P. 51–60. (In Russian).
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8. Gnecchi M1, Melo LG.Bone marrow-derived mesenchymal stem cells: isolation, expansion,
characterization, viral transduction, and production of conditioned medium. Meth. Mol. Biol. 2009, V. 482, P. 281–294. https://doi.org/10.1007/978-1-59745-060-7_18
9. Dhanasekaran M., Indumathi S., Poojitha R., Kanmani A., Rajkumar J.S., Sudarsanam D.
Plasticity and banking potential of cultured adipose tissue derived mesenchymal stem cells. Cell Tissue Bank.2013, 14(2), 303–315. https://doi.org/10.1007/s10561-012-9311-7
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11.Manini I., Gulino L., Gava B., Pierantozzi E., Curina C., Rossi D., Brafa A., D’Aniello C., Sorrentino V.Multipotent progenitors in freshly isolated and cultured human mesenchymal stem
cells: a comparison between adipose and dermal tissue. Cell Tissue Res. 2011, 344(1), 85–95.
https://doi.org/10.1038/emboj.2012.301
12. Romanov Y. A., Svintsitskaya V. A., Smirnov V. N. Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord. Stem Cells. 2003, 21(1), 105–10. https://doi.org/10.1634/stemcells.21-1-105
13. Fukuchi Y., Nakajima H., Sugiyama D., Hirose I., Kitamura T., Tsuji K. Human placenta-derived cells have mesenchymal stem/progenitor cell potential. Stem Cells.2004, 22(5), 649–58. https://doi.org/10.1634/stemcells.22-5-649
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Acad. Sci. 2008, V. 1127, P. 106–15. https://doi.org/10.1196/annals.1434.014
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Austr. New Zealand J. Experimental articles 81Obstetrics Gynecol. 2006, V. 46, P. 250–253.
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2007, V. 5, P. 57. https://doi.org/10.1186/1479-5876-5-57
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2006, 8(4), 315–317. https://doi.org/10.1080/14653240600855905
20. Prockop D., Phinney D., Blundell B. Mesenchymal stem cells: methods and protocols. Meth. Mol. Biol 2008, V. 449, P. 192.
21. Gimble J. M., Guilak F.Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 2003, 5(5), 362–369. https://doi.org/10.1080/14653240310003026
22. Baer P., Geiger Н.Adipose-Derived Mesen chymal Stromal/Stem Cells: Tissue Localization,
Characterization, and Heterogeneity.Stem Cells Int. 2012, V. 2012, P. 1–11.
https://doi.org/10.1155/2012/812693
23. Timper K., Seboek D., Eberhardt M. “Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells. Biochem. Biophys. Res. Commun. 2006, 341(4), 1135–1140. https://doi.org/10.1016/j.bbrc.2006.01.072
24. Pittenger M., Mackay A., Beck S., Jaiswal R., Douglas R. Multilineage Potential of Adult
Human Mesenchymal Stem Cells. Science. 1999, 284(5411), 143–147. https://doi.org/10.1126/science.284.5411.143
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2007, 212(3), 702–709.
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"Biotechnologia Acta" V. 11, No 1, 2018
Р. 70-75, Bibliography 10, English
Universal Decimal Classification: 616.096.578.828
https://doi.org/10.15407/biotech11.01.070
T. Y. Trokhymchuk 1, L. A. Ganova 2, N. Ya. Spivak 2
1 PJSC “SPC “Diaproph-Med”, Kyiv
2 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
The purpose of the work was to study the ability of test systems DIA-HIV 1/2 and DIA-HIV-Ag/Ab produced by PJSC “SPC “Diaproph-Med” to detect HIV infection. Reference blood serum of HIV-positive patients was tested using immunoassay analysis. It was shown that early detection of HIV infection is more effective using test kits of the fourth generation, which should be used for primary screening for HIV, and third-generation test systems should be used to confirm the positive results of primary analysis.
Key words: ELISA, early diagnosis of HIV, enzyme immunoassay test systems.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. HIV infection in Ukraine. Newsletter. Kyiv: Ministry of Health of Ukraine. 2016, 46, 7–14. (In Ukrainian).
2. Laboratory testing for the diagnosis of HIV infection. CDC. National Center for HIV/AIDS. 2013, 234.
3. Fiebig E. W., Wright D. J., Rawal B. D. Dynamics of HIV viremia and antibody seroconversion in plasma donors: implications for diagnosis and staging of primary HIV infection. AIDS. 2003. 17 (13), 1871?1879. https://doi.org/10.1097/01
4. Kislih O. M. Application of combined antigenantibody tests for immuno-enzymatic diagnosis of HIV infection. Lab. dіagnostika. 2003. N 4, P. 37–42. (In Ukrainian).
5. Pokrovsky V. V., Ermak T. Y., Beljaeva V. V., Yurin O. G. HIV-infection: clinics, diagnostics and treatments. Мoskva: Geotar Medic. 2000, 489.
6. National Program of the Ministry of Health of Ukraine. Order dated December 21, 2010 No 1141. (In Ukrainian).
7. Kotova N. V., Babіj N. O., Andrіanova І. V., Lyul’chuk M. G., Ringach N. O. Assessment of the current state of early diagnosis of HIV in children born to HIV-positive mothers. Kyiv: PC «Folіant», 2013,60. (In Ukrainian).
8. Lapach S. N., Gubenko A. E., Babich P. N. Statistical methods in biomedical research using Excel. Kyiv: Morion. 2002, 407. (In Russian).
9. Hemelaar J., Gouws E., Ghys P. D., Osmanov S. WHO-UNAIDS Network for HIV Isolation and Characterisation: Global trends in molecular epidemiology of HIV-1 during 2000– 2007. AIDS. 2011 (25), 679. doi: 10.1097/QAD. 0b013e328342ff93.
10. Zhang M., Foley B., Schultz A. K., Macke J. P., Bulla I., Stanke M., Morgenstern B., Korber B., Leitner T. The role of recombination in the emergence of a complex and dynamic HIV epidemic. Retrovirology. 2010, 7, 25. https://doi.org/10.1186/1742-4690-7-25
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"Biotechnologia Acta" V. 11, No 1, 2018
Р. 64-69, Bibliography 13, English
Universal Decimal Classification: 577.115:663.12
https://doi.org/10.15407/biotech11.01.064
1 National University of Food Technologies, Kyiv, Ukraine
2 SE “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”, Kyiv
The aim of the research was to study the accumulation of yeast lipids in the process of cultivation of strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 with the use of peat as growing substrate. The object of researches was strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 from “Collection of strains of microorganisms and plant lines for food and agricultural biotechnology” of SE “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”. As a raw material, lowland peat which was preliminarily processed with the help of cavitation or explosive autohydrolysis was used. The accumulation of lipids over the course of cultivation P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 on non-food substrate ? lowland peat was shown. The effect of pulp explosive autohydrolysis and pulp cavitation processing on biomass accumulation and lipids synthesis by strains P. аnomala IMB Y-5067 and R. gracilis IMB Y-5075 was researched. It was found that the maximum lipids accumulation by strains P. аnomala IMB Y-5067 (9.7 g/dm3) and R. gracilis IMB Y-5075 (8.9 g/dm3) was over the course of cavitation processing of peat pulp and additional application of salts and yeast extract into cultivation environment.
Key words: peat, Pichia anomala, Rhodotorula gracilis, yeast, cultivation, lipids.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017
References
1. Chuchuy V. P., Uminskiy S. M., Inyutin S. V. Alternative Energy Sources. Odesa: TES. 2015, 494 p. (Іn Ukrainian).
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3. Chuprina L. A. Organic waste recycling: Ukrainian technologies. Shlyahi rozvitku ukrayinskoyi nauki. 2016, V. 1, P. 115–117. (Іn Ukrainian).
4. Tigunova O. O., Beiko N. E., Kamenskyu D. S., Tkachenko T. V., Yevdokymenko V. O., Kashkovskiy V. I., Shulga S. M. Lignocellulosic biomass after explosive autohydrolysis as substrate for butanol obtaining. Biotechnol. acta. 2016, 9 (4), 28?34. https://doi.org/10.15407/biotech9.04.028
5. Saranchuk V. I., Ilyashov M. O., Oshovskii V. V., Biiletskii V. S. Fundamentals of Chemistry and Physics of Combustible Minerals. Donetsk: Shidnyi vydavnychyi dim. 2008, 640p. (Іn Ukrainian).
6. Ba?mler E. R. Solvent extraction: kinetic study of major and minor compounds. JAOCS. 2010, V. 87, P. 1489–1495.
7. Lakin G. F. Biometrics. Moskva: Vyisshaya shkola. 1990, 300 p. (Іn Russian). Experimental articles 69.
8. Veshnyakov V. A., Habarov Yu. G., Kamaki na N. D. Comparison of methods for the deter mi na tion of reducing substances: the Bertrand method, ebulliostatic and photometric methods. Khimiya rastitelnogo syrya. 2008, 4, 47–50. (Іn Russian).
9. Gerasimov Yu. Yu., Hlyustov V. K. Mathematical methods and models in computer calculations. Moskva: MGUL. 2001, 260 p.
10. Verhoeven Jos T. A., Setter Tim L. Agricultural use of wetlands: opportunities and limitations. Ann Bot. 2010, 105 (1), 155–163. dhttps://doi.org/10.1093/aob/mcp172
11. Tkachenko A. F., Tigunova E. A., Shulga S. M. Microbial lipids — an alternative source of raw materials for biofuels. Mikrobiologiya i biotekhnologiya. 2012, V. 3, P. 17–33. (Іn Russian).
12. Tkachenko A. F., Tigunova E. A., Shulga S. M. Lipids of microorganisms as a source of biofuel. Tsitologiya i genetika. 2013, 6 (47), 22–29. (Іn Russian).
13. Shulga S. M., Tkachenko A. F., Beyko N. E., Homenko A. I., Andriyash A. S. Biosynthesis of lipids by yeast Rhodotorula gracilis. “Biotekhnolohiia”. 2010, 3 (3), 58–65. (Іn Russian).
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"Biotechnologia Acta" V. 11, No 1, 2018
Р. 58-63, Bibliography 17, English
Universal Decimal Classification: 576.858
https://doi.org/10.15407/biotech11.01.058
POLYMERASE CHAIN REACTION FOR IDENTIFICATION OF CYPRINID HERPESVIRUSES IN UKRAINE
Yu. P. Rud 1, M. I. Maistrenko 2, L. P. Buchatskiy 1, 2
1 Institute of Fisheries of the National Academy of Agriculture Sciences of Ukraine, Kyiv
2 ESC Institute of Biology and Medicine, Taras Shevchenko Kyiv National University, Ukraine
The aim of the research was to investigate diseased fish species Labeo (Labeo bicolor) and Sack-gill Catfish (Heteropneustes fossilis) by testing several diagnostic systems targeting fish herpesviruses in purpose to determine the etiology of the outbreak infection in aquarium fishes at the Kуiv Zoo during summer 2017. During a fish health inspection of aquariums in Kyiv Zoo, an Cyprinid herpesvirus 3 ? CyHV-3 was detected in Labeo and Sack-gill Catfish. Preliminary examination of infected fish revealed a range of lesions particularly in internal organs and tissues. The gills of diseased fish were characterized by hyperaemia and necrosis. The infringement of liver color and structure, kidney swelling and gallbladder necrosis were observed in both fish species. The virus did not grow in fish cell lines of RTG-2, FHM and EPC, that was evident by absence of any morphological changes in appropriate cell lines. Also initially fish were tested for parasitic and bacterial infections and they were determined to be non-infected. Our results demonstrated that specific oligonucleotide primers for thymidine kinase gene of CyHV-3 were successfully amplified the specific DNA fragments. The length of polymerase chain reaction product, as expected, was 264 nucleotide pairs. The amplified specific fragments were identical to the area of thymidine kinase gene CyHV-3, as was shown by sequence analysis. The identity of nucleotide sequences composed 97?99%. The same positive results were obtained using primers that recommended by the International Epizootic Bureau, fragments in size of 409 and 292 were also obtained. In our opinion, CyHV-3 was brought to Ukraine by import of aquarium fish avoiding sanitary control of transboundary transportation. Therefore, the uncontrolled import of aquarium and cultured fish species is a serious problem because imported fish could be as a source of highly pathogenic infections for industrial aquaculture.
Key words: polymerase chain reaction, CyHV-3 fish herpesvirus of Labeo bicolor and Sack-gill Catfish.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. Waltzek T. B., Kelley G. O., Alfaro M. E., Kurobe T., Davison A. J., Hedrick R. P. Phylogenetic relationships in the family alloherpesviridae. Dis. Aquat. Org. 2009, V. 84, P. 179–194.
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5. Waltzek T. B., Kelley G. O., Stone D. M., Way K., Hanson L., Fukuda H., Hirono I., Aoki T., Davison A. J., Hedrick R. P. Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae. J. Gen. Virol. 2005, V. 86, P. 1659–1667.
6. Hedrick R. P., Gilad O., Yun S., Spangenberg J. V., Marty G. D., Nordhausen R. W., Kebus M. J., Bercovier H., Eldar A. A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp. J. Aquat. Anim. Health. 2000, V. 12, P. 44–57.
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