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- Hits: 620
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 12, No 4, 2019
Р. 57-64, Bibliography 33, English
Universal Decimal Classification: 547.458.1;612.115.12
https://doi.org/10.15407/biotech12.04.057
THE MECHANISM OF CLOTS FORMATION IN BLOOD PLASMA UNDER THE ACTION OF CHITIN DERIVATIVES
1National University of Life and Environmental Scienсes of Ukraine, Kyiv
2Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv
The aim of the research was to find out the clot formation by the action of chitin derivates. The biochemical and immunologic investigation methods such as obtaining of fibrinogen, chitosan derivates, electrophoresis in PAAG, Western blot analysis, ELISE, the activated partial thromboplastin time and prothrombin time coagulological tests were used in these studies. The next results were obtained: chitin derivatives in equal measure cause the clot formation in whole blood, blood plasma and fibrinogen solution; the fibrinogen precipitate formed as a result of their action, practically does not contain fibrin; chitosan does not cause the activation of coagulation factors and the absence of newly-formed fibrin confirms it; the addition of calcium chloride to fibrinogen solution in concentration-dependent manner inhibits effect of chitosan.
Thus, under the action of chitosan, a fibrinogen precipitate forms due to the destabilization of its molecule by the lack of calcium. Absence of fibrin degradation products excludes the possibility of the fibrinolytic system activation and physiological degradation of the clot. It makes no sense to use haemostatic drugs based on chitosan in clinical practice.
Ключевые слова: chitin and its derivates, fibrinogen, haemostasis system.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019
References
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 12, No 4, 2019
Р. 50-56, Bibliography 20, English
Universal Decimal Classification: 628.3
https://doi.org/10.15407/biotech12.04.050
Sablii L., Korenchuk M., Kozar M.
The National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute", Kyiv
The aim of the research was to determine the degree of influence of nitrate wastewater concentration on the process of removal of phosphorus compounds by sequential water treatment in anoxic and aerobic conditions in an activated sludge system. Model wastewater solutions were used for research with following parameter: biochemical oxygen demand for 20 days –200 mgО2/l; phosphate concentrations – 11.87–12.38 mg/l; nitrate concentrations – 21.0; 36.0 and 48.0 mg/l. Аctivated sludge was added to them with content in solutions 2.2 mgО2/l to provide biological processes. For simulation of the biological process of dephosphotation in wastewater with usage of activated sludge in sequentially formed anoxic and aerobic conditions, a model sequential reactor — SBR reactor — was used. As the results show, with the increase in the concentration of nitrates at the inlet from 21.0 to 48.0 mg/l, the phosphate concentration in the treated solutions at the outlet from the bioreactor increases by 7.3%. Thus, from the work presented here, it can be concluded that for successful and effective implementation of the dephosphotation process the elimination of the nitrate present in wastewater is required. It is reasonable to separate processes of denitrification and dephosphotation in separate structures with the provision of minimal nitrate influence on the phosphorus removal from wastewater.
Key words: phosphate, nitrate activated sludge, wastewater.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019
References
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 12, No 4, 2019
Р. 42-49, Bibliography 23, EnglishUniversal Decimal Classification:
577.121.7/ 612; 618.3-57.04; 57.017.3
https://doi.org/10.15407/biotech12.04.042
DYNAMICS OF BRAIN ENZYMES ACTIVITY IN RAT EXPOSED TO HYPOXIA
Rashidova А. M., Babazadeh, S. N. Mammedkhanova V. V., Abiyeva E. Sh.
Academician Abdulla Garayev Institute of Physiology, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
The aim of the work was to study the dynamics activity of lactate dehydrogenase (LDH; EC 1.1.1.27), aconitase (AH; EC 4.2.1.3), NAD-dependent malate dehydrogenase (MDH; EC 1.1.1.37), succinate dehydrogenase (SDH; EC 1.3.99.1) in homogenates and sub-fractions of brain structures of rat prenatally endured hypoxia at the organogenesis stage (in 11–15 days of development) and their role in the formation of compensatory - adaptive mechanisms in brain in postnatal ontogenesis. It was revealed that increasing of lactate dehydrogenase and malate dehydrogenase activity (P<0.001; P<0.01, correspondently) in the brain structures of the rats prevented metabolic disturbances in the regulation mechanisms of biosynthetic and bioenergetics processes in the brain. It has been shown that prenatal hypoxia upregulates aconitase activity in postnatal development and this process, probably, has a reversible character (P<0.01), the highest indices of succinate dehydrogenase activity were noticed in the hypothalamus and cerebellum of 30-day-old rat as compared to the other structures (P<0.001). Based on the data obtained, it can be concluded that hypoxia at the stage of organogenesis leads to a change in the energy supply process of the brain structures and, possibly, is irreversible. Analysis of changes in the enzymatic system in ontogenesis allows us to identify adaptation mechanisms and to assess the dynamics of changes in enzyme activity when the functional state changes, which make it possible to identify adaptive reserves of enzymes LDH, AH, MDH and SDH in brain exposed to hypoxia.
Key words: enzymes of energy metabolism.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019
References
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 12, No 4, 2019
Р. 34-41, Bibliography 20, English
Universal Decimal Classification: 579.22: 582.28
https://doi.org/10.15407/biotech12.04.034
1Poyedinok N. L., 2Tugay T. I., 2Tugay A. V., 3Mykchaylova O. B.,4Sergiichuk N. N., 5Negriyko A. M.
1 Institute of Food Biotechnology and Genomicsof the National Academy of Sciences of Ukraine, Kyiv
2 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
3 Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kiyv
4 Open International University of Human Development "Ukraine", Kyiv
5 Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv
The aim of the work was to study the effect of nitrogen concentrations on photo-induction of growth, enzymatic activity and synthesis of melanin by the medicinal fungus Inonotus obliquus (Ach.: Pers.) Pil?t from the Collection of cultures of cap mushrooms of Kholodny Institute of Botany of the National Academy of Sciences of Ukraine. Irradiated by light of low intensity, different coherence and in different wavelength ranges, mycelium was cultivated in a dynamic mode on a glucose-peptone medium with different concentrations of total nitrogen. The concentration of the nitrogen source was not shown to significantly affect the photo-induced stimulation of the growth of I. obliquus. The increase in biomass accumulation of mycelium photoactivated in different modes was almost the same in all variants of the experiment, compared with the biomass of not-irradiated mycelium. A reliable dependence of the photo-stimulation of melanin synthesis on the concentration of nitrogen in the medium was established. Reduced nitrogen concentration more than twice increased the stimulating effect of low-intensity laser radiation with a wavelength of 488 nm. Using substrate with a reduced content of the nitrogen source is advisable to increase the photo-induced stimulating effect in the production of extracellular catalase, tyrosinase and polyphenol oxidase, intracellular peroxidase.
Thus, the cultivation parameters of I. obliquus and the light treatment regimes of the inoculum should be adjusted according to the target bioactive components.
Key words: Inonotus obliquus, photoinduction, nitrogen, melanin, catalase, tyrosinase, polyphenol oxidase, peroxidase, growth activity.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019
References
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- Details
- Hits: 717
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 12, No 4, 2019
Р. 27-33, Bibliography 20, English
Universal Decimal Classification: 633.584.5+631.427.1
https://doi.org/10.15407/biotech12.04.027
1Kryvtsova M., 1Bobryk N., 2Simon L.
1 Uzhhorod National University, Ukraine
2 University of Ny?regyh?za, Hungary
The aim of the work was to study the soil agrochemical indices, soil microbiocoenosis, in case of growing of energy cultures and based on the mineralization coefficient, to make a conclusion on the speed of mineralization processes in the soils under study. In conditions of continuous field experiment (2011–2016), the dynamics of soil microbial associations was studied for willow (Salix triandra x Salix viminalis 'Inger') cultivation with application of experimental fertilizers of different types. In the research fertilizers there were used sulfuric urea, municipal biocompost, municipal sewage sludge compost, rhyolite tuff and willow ash. The soil microbiotic communities analysis was conducted by the method of serial dilutions of soil suspension with the use of differentially diagnostic nutrient media: meat-peptone agar, starch-ammonium agar, Ashby medium, potato agar, Czapek Dox medium, starvation agar, Ploskirev medium. The direction of the microbiological processes in the soils determined.
According to the results, it was established that the most promising for the purpose of improving the metabolic activitiof the soil (in the growth of energy willow) is a municipal sewage sludge compost and a municipal biocompost. In case of the use of municipal sewage sludge compost, the number of intestinal bacteria, ammonifiers, micromycetes and actinomycetes was doubled as compared with the control. In case of the use of municipal biocompost, the levels of microscopic fungi and cellulolytic bacteria doubled, and those of intestinal bacteria and pedotrophs tripled as compared with the control. While calculating the mineralization/immobilization index, it was shown that the most significant deviation from the control plot was found in the rhyolite tuff treated soil – a decrease by 6 times, and in case of willow ash by 2.3 times, which proved the inhibition of mineralization of the organic substances in the soil.
Key words: energy willow, organic and inorganic soil additives, soil microbiocoenosis, mineralization coefficient.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019
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