6_2016(en)

Details: Hits: 357

"Biotechnologia Acta" V. 9, No 6, 2016
https://doi.org/10.15407/biotech9.06.082
Р. 82-89, Bibliography 16, English
Universal Decimal Classification: 577.3.04

6 2016

THE IMPACT OF SUPERHIGH FREQUENCY ELECTROMAGNETIC RADIATION ON THREONINE PRODUCER Brevibacterium flavum

L. V. Marynchenko¹, O. I. Nizhelska², D. M. Lytvynenko¹, G. M. Zabolotna³

¹National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute»
²The Scientific and Training Centre “Physical and Chemical Material Science”
³Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

The aim of the research was the impact of non-thermal electromagnetic radiation of superhigh frequencies with waves of millimeter range on threonine amino acid synthesis by bacteria Brevibacterium flavum for ordinary (not mutant) and mutant strains.

The frequencies of millimeter range waves were selected according to previous works as 41.76; 42.2 and 61.0 GHz. The exposition was 10 min. The control samples of bacterial suspension in the flasks were kept under the same conditions as the test ones. Irradiated suspensions were used as inoculum for fermentation on molasses wort at t = +30 ?C with aeration. After cultivation for 3 days the samples irradiated with frequency 42.2 and 61.0 GHz gave an increase in colonies forming units, respectively, 1.4 and 1.9 times compared to the control for the non-mutant strain. The quantity of synthesized threonine was determined by thin-layer chromatography on the plates of ciluprevir. A significant increase of the threonine content in the culture fluid was observed for the non-mutant strain (70% compared to control) after the irradiation with frequency 61.0 GHz. The splitting of the colonies planted pigmentation was observed: the control samples were mostly pigmentated, and irradiated bacteria lost this ability immediately after exposure, but after the culturing the irradiated samples restored pigmentation. The pigmentation ability was confirmed by the data on the accumulation threonine in the culture fluid.

The Brevibacterium flavum mutant strain did not respond to the irradiation, this influence was negative for generative abilities and accumulation of threonine in the culture fluid.

Ключевые слова: Brevibacterium flavum, threonine, nonthermal electromagnetic radiation of millimeter range waves.

References

1. Andriiash G. S., Zabolotna G. M., Tkachenko A. F., Blume Ya. B., Shulga S. M. Threonine synthesis of Brevibacterium flavum mutant strain. Threonine: Food Sources, Functions and Health Benefits. 2015, (Nova), P. 1–26.

2. Andriyash G. S., Zabolotna G. M., Shulga S. M. Auxotrophity of producents of lysine. Biotechnologiya. 2012, 5 (1), 70–77.

3. Andriyash G. S., Zabolotnа G. M., Shulga S. М. The mutant strains of microorganisms ? producers of lysine and threonine. Biotechnol. аcta. 2014, N 3, P. 95–101. https://doi.org/10.15407/biotech7.03.095

4. Shulga S. M., Tigunova O. O., Tkachenko A. F., Beyko N. E., Andriash G. S., Priemov S. G. Threonine biosynthesic intensification by Brevibacterium flavum ТН-7 strain. Biotechnologia. 2011, N 5, P. 97–103. (In Ukraine).

5. Grundler W., Keilmann F., Putterlik V., Santo L., Strube D., Zimmermann I. Nonthermal resonant effects of 42 GHz microwaves on the growth of yeast cultures. In: Frohlich H., Kremer F. (eds.) Coherent excitations in biological systems. Springer, Berlin Heidelberg New York. 1983, P.21–37. https://doi.org/10.1007/978-3-642-69186-7_4

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7. Kalinichenko S. V. The influence of millimeter electromagnetic waves high frequency range on the adgesive properties of Corynebacteria. The Journal of V. N. Karazin Kharkiv National University. 2004, 9 (639), 7–11.

8. Webb S. J. Nonlinear Phenomenain Bioenergetics and Oncology as seen as 25 years of research with mm microwaves and Raman spectroscopy. Nonlinear Electromagnetics in Biological Systems. Ed. by W. R. Adey. New York, London, Plenum Press. 1984, 603 p.

9. Jankovi? S. M., Milo?ev M. Z., Novakovi? M. L. The Effects of Microwave Radiation on Microbial Culture Hospital Pharmacology. 2014, 1 (2), 102–108.

10. Marynchenko L. V., Nizhelska O. I., Marynchenko V. O. Stimulation of Accumulation ofBiomass and Fermenting Activity of Saccharomyces cerevisiae Yeast by Extra High Frequency Electromagnetic Irradiation. Research Bulletin of Ukraine Kyiv Polytechnic Institute. 2011, 3 (77), 68–73.

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13. Grundler W., Keilmann F. Sharp resonance in yeast growth prove nonthermal sensitivity in microwaves. Phys. Rev. Lett. 1983, V. 51, P. 1214–1216. https://doi.org/10.1103/PhysRevLett.51.1214

14. Yakunov A. V., Nizhelska A. I., Marinchenko L. V., Marinchenko V. A., Makara V. A. Influence of Processing of Yeast Saccharomyces cerevisiae with Millimeter Waves on Fermentation Indicesin Technology of Bioethanol Production. Surface Engineering and Applied Electrochemistry. 2015, 51 (2), 156–161. https://doi.org/10.3103/S1068375515020143

15. Andreev E. A., Belyi M. U., Ivanchenko I. A., Yakunov A. V. Determination of threshold power of electromagnetic field of millimeter range affecting thegrowth of yeast cells. Elektron. Obrab. Mater. 1990, N 1, P. 61–63.

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"Biotechnologia Acta" V. 9, No 6, 2016
https://doi.org/10.15407/biotech9.06.072
Р. 72-81, Bibliography 42, English
Universal Decimal Classification:60-022.532:578.62+546.65

6 2016

in vivo EFFECTS OF RARE-EARTH BASED NANOPARTICLES ON OXIDATIVE BALANCE IN RATS

V. K. Klochkov ¹, V. P. Kaliman ², N. A. Karpenko ³, N. S. Kavok ¹, M. Yu. Malyukina ¹, S. L. Yefimova ¹, Y. V. Malyukin ¹

¹ Institute for Scintillation Materials of the National Academy of Sciences of Ukraine, Kharkiv
² Kharkiv National Medical University, Ukraine
³ SI “Danilevsky Institute for Endocrine Pathology Problems of the National Academy of Medical Sciences of Ukraine”, Kharkiv

The purpose of the research was to find the influence of rare-earth based nanoparticles (CeO₂, GdVO₂: Eu³⁺) on the oxidative balance in rats. We analyzed biochemical markers of oxidative stress (lipid peroxidation level, nitric oxide metabolites, sulfhydryl groups content) and enzyme activities (superoxide dismutase, catalase) in tissues of rats. It has been found that administration of both types of the nanoparticles increased nitric oxide metabolites and products of lipid peroxidation in liver and spleen within 5 days. At injections of GdVO₂: Eu³⁺ lipid peroxidation products, nitric oxide metabolites in serum at 5, 10 and 15 days of the experiment was also increased whereas the level of sulfhydryl groups decreased compared to the intact state and the control. In contrast, under the influence of nanoparticle CeO₂ level diene conjugates were not significantly changed and the level of nitric oxide metabolites within 15 day even decreased. During this period, under the influence of both types of nanoparticles the activity of superoxide dismutase was increased, catalase activity was not changed. Oxidative stress coefficient showed the less pronounced CeO₂ prooxidant effect (2.04) in comparison to GdVO₂: Eu³⁺ (6.89). However, after-effect of both types of nanoparticles showed complete restoration of oxidative balance values.

Ключові слова: nanoparticles CeO₂ and GdVO₄:Eu³⁺ , oxidative balance.

References

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"Biotechnologia Acta" V. 9, No 6, 2016
https://doi.org/10.15407/biotech9.06.058
Р. 58-71, Bibliography 13, English
Universal Decimal Classification: 504.064.3:574 (477.42)

HYDROPHYTE WATER PURIFICATION UNDER CONDITIONS OF “ZHITOMYRVODOKANAL” COMMUNAL ENTERPRISE

L. D. Romanchuck, T. P. Fedonyuk, R. G. Fedonyuk, А. A. Petruk

Zhytomyr National Agroecological University, Ukraine

The aim of the research was the hydrophyte water purification method testing, water purification effect determination, also of the allowable loads of the most toxic pollutants on higher aquatic plants under conditions of modeling laboratory systems and advanced ways of processed phytomass use.

The results of hydrophyte water purification are presented, different types of higher aquatic plants under laboratory systems of water quality formation impact are analyzed, the water purification effect and the prospective ways of phytomass waste use are determined. The hydrophyte load use according to all studied variants showed a positive trend concerning the improvement of all the water quality indicators studied, and the cleaning from pollutants effect according to some indicators was more than 80%. The aquatic plants biomass can be used not only for obtaining from meliorant wastewater but also in various economic sectors – as feed additive for farm animals.

Ключові слова: phytomelioration, hydrophytes, purification effect, chemical pollution, domestic waste water.

References

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Details: Hits: 206

"Biotechnologia Acta" V. 9, No 6, 2016
https://doi.org/10.15407/biotech9.06.050
Р. 50-57, Bibliography 21, English
Universal Decimal Classification: 759.873.088.5:661.185

6 2016

CALCIUM AND MAGNESIUM CATIONS INFLUENCE ON ANTIMICROBIAL AND ANTIADHESIVE ACTIVITY OF ACINETOBACTER CALCOACETICUS ІMV B-7241 SURFACTANTS

P. Pirog, I. V. Sidor, D. A. Lutsai

National university of food technology, Kiyv, Ukraine

The aim of the work was to study the effect of calcium and magnesium cations on NADP⁺-dependent glutamate dehydrogenase activity (key enzyme of biosynthesis of Acinetobacter calcoaceticus ІMV B-7241 surface-active aminolipids) followed by modification of medium composition and determining antimicrobial and antiadhesive activity of synthesized surfactants.
The strain IMV B-7241 was grown in medium with ethanol. NADP⁺-dependent glutamate dehydrogenase activity of the cell-free extract was analyzed using the formation of glutamate in the oxidation of NADPH. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). Antimicrobial against bacteria properties of the surfactants were determined by index of the minimal inhibitory concentration. The number of attached cells and the degree of biofilm destruction were analyzed spectrophotometrically.

It was established that in the presence of 10 mM Cа²⁺ and Mg₂ NADP⁺-dependent glutamate dehydrogenase activity in the cell-free extract increased to 1.5 times in comparison with that without cations. Increasing concentration of magnesium sulfate to 0.2 g/l, or adding CaCl2 (0.1 g/l) into cultivation medium of IMV B-7241 strain was accompanied by rise of NADP⁺-dependent glutamate dehydrogenase activity in 2.4 and 3.0 times respectively, as well as increasing antimicrobial and antiadhesive activity of synthesized surfactants. Minimal inhibitory concentration of surfactants synthesized in modified media against some bacteria was in 1.3?3.5 times, adhesion on abiotic surfaces treated with such surfactants in an average of 7?13% lower, and the degree of biofilm destruction in 7?13% higher as compared to indicators for the surfactant produced in the base medium.

The obtained results indicated the possibility of regulating antimicrobial and anti-adhesive activity of surfactants under producer cultivation.

Ключові слова: Acinetobacter calcoaceticus IMV B-7405, surfactants, NADP⁺-dependent glutamate dehydrogenase activity, calcium and magnesium cations.

References

1. Pirog T. P., Savenko I. V., Shevchuk T. A. Effect of cultivation conditions of Acinetobacter calcoaceticus ІMV B-7241 on surfactants antiadhesive properties. Microbiol. Zh. 2016, 78 (1), 2?12. (In Russian).

2. Pirog T. P., Savenko I. V., Shevchuk T. A., Krutous N. V., Iutynska G. O. Antimicrobial properties surfactants synthesized under different cultivation conditions of Аcinetobacter calcoaceticus IMV B-7241. Microbiol. Zh. 2016, 78 (3), 2–12. (In Ukrainian).

3. Pirog T. P., Shevchuk T. A., Mashchenko O. Yu., Parfenyuk S. A., Iutinskaya G. A. Еffect of growth factors and some microelements on biosurfactant synthesis of Аcinetobacter calcoaceticus IMV B-7241. Microbiol. Zh. 2013, 75 (5), 19?27. (In Russian).

4. Pirog T. Р., Konon A. D. Microbial surfactants. I. Glycolipids. Biotechnol. аcta. 2014, 7 (1), 9–30. . (In Ukrainian). https://doi.org/10.15407/biotech7.01.009

5. Cochrane S. A., Vederas J. C. Lipopeptides from Bacillus and Paenibacillus spp.: a gold mine of antibiotic candidates. Med. Res. Rev. 2016, 36 (1), 4–31. https://doi.org/10.1002/med.21321

6. Bernat P., Paraszkiewicz K., Siewiera P., Moryl M., P?aza G., Chojniak J. Lipid composition in a strain of Bacillus subtilis, a producer of iturin A lipopeptides that are active against uropathogenic bacteria. World J. Microbiol. Biotechnol. 2016, 32 (10). https://doi.org/10.1007/s11274-016-2126-0

7. Abalos A., Pinazo A., Infante M. R., Casals M., Garc?a F., Manresa A. Physicochemical and antimicrobial properties of new rhamnolipids produced by Pseudomonas aeruginosa AT10 from soybean oil refinery wastes. Langmuir. 2001, 17 (5), 1367?1371. https://doi.org/10.1021/la0011735

8. Mandal S. M., Barbosa A. E., Franco O. L. Lipopeptides in microbial infection control: scope and reality for industry. Biotechnol. Adv. 2013, 31 (5), 338?345.
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Details: Hits: 272

"Biotechnologia Acta" V. 9, No 6, 2016
https://doi.org/10.15407/biotech9.06.039
Р. 39-49, Bibliography 28, English
Universal Decimal Classification: 577.114.083:543.544.153:543.545.2

6 2016

HONEYBEE (Apis mellifera) CHITOSAN: PURIFICATION, HETEROGENEITY AND HEMOCOAGULATING ACTIVITY

M. D. Lootsik ¹, R. O. Bilyy², M. M. Lutsyk², N. O. Manko ³, S. A. Navytka ³, V. I. Kutsiaba ¹, R. S. Stoika ¹

¹Institute of Cell Biology of the National Academy of Sciences of Ukraine, Lviv
² Danylo Halytsky Lviv National Medical University, Ukraine
³Ivan Franko National University, Lviv, Ukraine

The aim of the research was to elaborate the method of chitosan preparation obtaining from honeybee corpses. It included the following stages: 1) washing of bee corpses with hot water; 2) delipidation of powdered material with petrol ether; 3) decalcification by EDTA at pH 4; 4) deproteination by 3 fold treatment with 5% NaOH 1 h at 70 ^oC; 5) bleaching of chitin with sodium hypochlorite; 6) deacetylation of chitin in 40% NaOH solution at 115 ^oC for 3 h; 7) purification of chitosan by its dissolving in 3% acetic acid and precipitation with ammonia at pH 8,5; 8) separation into three fractions precipitated at pH 6.4, 7.0 and 8.6. The yield of chitosan from dry bee powder was 8.5–10.0. A distinct diversity in the molecular mass of different chitosan fractions was revealed in the range of 80–320 kDa. Heterogeneity of chitosan samples was studied using gel permeation chromatography on Acrylex P-150 and electrophoresis in a slab of polyacrylamide gel with a stepwise gradient of acrylamide concentration 5, 10, 15, 20% in pH 4.5 buffer system. High molecular mass chitosan possessed blood coagulating activity, while low molecular mass fractions were poorly active. The rate of blood clot formation induced by active honeybee chitosan was 3 times lower comparing with that of chitosans obtained from crabs or shrimps.

Ключевые слова: bee subpestilence, chitosan.

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