1_2009(en)

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 117-123, Bibliography 11, Ukrainian.
Universal Decimal classification 541.18.045+577.15

α-AMILASE IMMOBILIZATION ON CELLULOSE MEMBRANES
MODIFIED BY CHITOSANE AND СIBACRON BLUE F3G-A

V. V. Konovalova, K. Е. Guzikevich, G. A. Pobigay, A. F. Burban, S. T. Oliynichuk

National University of Kiev Mohyla-Academy, Membrane Research Center, Kуiv
Ukrainian research institute of alcohol and biotechnology of foods, Kyiv

A method of the affinity membranes producing based on chitosane covalent bonding with cellulose membranes surface and Cibacron Blue F3G-A dye fixing as affine ligand has been developed. Transport and surface properties of the modified membranes have been studied. Optimum α-amilase immobilization is found to be the following: pH and solution concentration are 3.5 and 1 mg/sm³ respectively. Starch conversion of unregenerated membranes with immobilized enzyme was up to 84%.

Key words: enzyme immobilization, α-amylase, affine membranes.

Details: Hits: 29

ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 9-23, Bibliography 87, Ukrainian.
Universal Decimal classification 577.152.34

GLUCOAMYLASE OF MICROORGANISMS. BIOSYNTHESIS, PROPERTIES, MECHANISM OF ACTION AND APPLICATION

N. V. Borzova, O. V. Gudzenko, L. D. Varbanets

Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv

Data on regular position of glucoamylase in modern classification are given in the review. Current views on regulation of microbial glucoamylases biosynthesis are stated, the issues concerning structure and functions of individual domains of enzyme molecule, their interrelation and role in catalysis are discussed.

Physical and chemical properties of fungal glucoamylases are detailed.Possibility of effective immobilization of enzymes on various carriers with the purpose of their using in various technological processes was shown.

Key words: glucoamylase, biosynthesis, active centre structure, mechanism of action, enzyme properties, glucoamylase immobilization, practical use.

Details: Hits: 31

ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 24-34, Bibliography 43, Ukrainian.
Universal Decimal classification: 577.322.7: 577.112.386.2

DISULFIDE BONDS IN STRUCTURAL AND FUNCTIONAL PROTEIN ORGANIZATION

L. P. Urvant, I. I. Patalakh

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv

Modern vision about disulfide bonds role in light of providing the structural and functional properties of blood plasma proteins is proposed. New approaches concerning recombinant and secretory proteins refolding are generalized. A problem concerning oxidative folding and disulfide bonds significance for secretory protein posttranslation ripening inside of endoplasmic reticulum with reductase enzyme system participation is discussed.

Key words: disulfide bonds, secretory proteins, plasminogen, oxidative folding, proteins renaturation.

Details: Hits: 36

ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 59-68, Bibliography 35, Ukraininan.
Universal Decimal classification: 577(с87.1+112.4)

CLONNING AND EXPRESSION OF FUNCTIONAL FRAGMENT D-E-A-A*
OF PROTEIN А FROM Staphylococcus aureus

O. S. Oliynyk, D. V. Kolibo, А. А. Kaberniuk, A. Yu. Labyntsev
N. V. Korotkevich, S. I. Romanyuk, S. V. Komisarenko

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

Recombinant fragment of protein A from Staphylococcus aureus with unique sequence, which has four domains D-E-A-A* and is expressed in Escherichia coli have been obtained. Conjugates of recombinant fragment with horse radish peroxidase and colloidal gold nanoparticles were used for testing its functional activity and immunoglobulin-binding properties.

It was shown, that recombinant fragment of protein A marked by horseradish peroxidase or colloidal gold nanoparticles, has the same specificity to mammal immunoglobulines as native protein A. Therefore an obtained analog of protein A can be used not only in development of chromatography sorbents for immunoglobulins purification and immunochemical conjugates for immunoglobulins detection, but also in fundamental investigations of immunobiological properties of protein A.

Key words: protein A, Staphylococcus aureus, recombinant analog, immunoglobulin-binding activity, immunoenzyme assay, immunochromatography tests.

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 46-58, Bibliography 88, English.
Universal Decimal classification 547.9:612.397:678.012

DIRECTED DRUG DELIVERY SYSTEMS. BIOTECHNOLOGICAL ASPECTS

O. Yu. Galkin, L. B. Bondarenko, A. S. Grishyna, O. M. Dugan

National Technical University of Ukraine «Kiev Polytechnic Institute», Kyiv
Institute of Pharmacology and Toxicology of AMS of Ukraine, Kyiv

Publications concerning methods of creation of the drug delivery systems based on liposomal forms and receptordependent endocytosis are summarized. The methods of use of the directed drug delivery systems in creation of anticancerogenical and antibacterial agents, vaccines and in gene therapy are examined.

Key words: directed drug delivery systems, pharmaceuticals, liposomes, endocytosis, anticancer agents, monoclonal antibodies.

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