ISSN 1995-5537
"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 88-98, Bibliography 24, Ukrainian.
Universal Decimal classification 577.15:573.6
Р. 88-98, Bibliography 24, Ukrainian.
Universal Decimal classification 577.15:573.6
OPTIMIZATION OF METHODS OF GLUCOSE CONTENT DETERMINATION IN WINE BY ENZYME AMPEROMETRIC BIOSENSOR
T. B. Goriushkina, О. V. Ostroukhova, О. P. Soldatkin, S. V. Dzyadevych
Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine,Kyiv
Taras Shevchenko National University, Kyiv
Institute for Vine and Wine «Magarach», Yalta
Taras Shevchenko National University, Kyiv
Institute for Vine and Wine «Magarach», Yalta
A method of glucose determination in wine by an amperometric biosensor on the basis of platinum printed electrode SensLab and glucose oxidase was optimized. Selectivity of glucose biosensors created on the basis of two different methods of glucose oxidase immobilization was investigated. Main wine interferents were demonstrated to have no effect on work of the biosensor with glucose oxidase immobilised in glutaraldehyde vapor, but they influenced on work of the biosensor with glucose oxidase immobilised in PEDT polymer. Reproducibility, operational and storage stability of the created biosensor on the basis of glucose oxidase immobilised in glutaraldehyde vapor were investigated. Glucose concentration in several types of wine and must was determined with the created biosensor, and the results were compared with those obtained by traditional analytical method — HPLC. Good correlation between data obtained by the biosensor method and HPLC was shown. It was also determined that biosensor with glucose oxidase in PEDT polymer is characterized by extremely wide dynamic range of work and it can be successfully used for glucose analysis in the liquids without ethanol and glycerol — for example, in blood.
Key words: amperometric biosensor, glucose, wine, must.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008