3_2008(en)

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ISSN 1995-5537

“Biotechnology” journal Vol. 1, No. 3, 2008
P. 64-71, Bibliography. 21, укр.
Universal Decimal classification: 577.15 + 543.6 + 543.9 + 543.55

NOVEL AMPEROMETRIC BIOSENSOR BASED ON GLYCEROL OXIDASE
FOR GLYCEROL DETERMINATION

T. B. Goriushkina, L. V. Shkotova, G. Z. Gayda, H. M. Klepach, M. V. Gonchar3,

O. P. Soldatkin, S. V. Dzyadevych

Institute of Molecular Biology and Genetics, of National Academy of Sciences of Ukraine, Kyiv
Taras Shevchenko Natoinal University, Kyiv
Institute of Cell Biology, of National Academy of Sciences of Ukraine, L’viv
Ivan Franko State Pedagogical University, Drogobych

The paper presents comparative effectiveness analysis of glycerol oxidase preparations, used for development of amperometric biosensor for glycerol determination, which are different in method of preparation and in characteristics.

Enzyme preparation, whose immobilization on transducer surface results in sensor demonstrating its best operational characteristics, was selected. PH-optimum for the operation of the developed amperometric biosensor was determined; the values of buffer volume and background electrolyte concentration in buffer were shown to have no effect on the work of glycerol biosensor. The analysis of glycerol concentration in sample solutions was carried out using the developed amperometric biosensor based on glycerol oxidase.

Key words: amperometric biosensor, glycerol oxidase, glycerol, immobilization, electrochemical polymerization.

Details: Hits: 26

ISSN 1995-5537

“Biotechnology” journal Vol. 1, No. 3, 2008
P. 56-63, Bibliography. 13, укр.
Universal Decimal classification: 581.143.6:576.5+663.1

GENERATION OF Echium plantagineum L. TISSUE CULTURE WHICH IS PRODUCENT OF SHIKONIN PIGMENTS

О. О. Poronniк, V. А. Shablij, V. А. Кunakh

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv

E. plantagineum tissue culture has been generated from the roots of seed shoots on 5C01 agarized nutrient medium. Cultivation on the LS-m nutrient medium induced biosynthesis of the red shikonin pigments. Variant of callus tissue (3Ep), accumulating 2.11% of shikonin derivatives per dry biomass whose yield on the 14th day of growth constituted up to 16 mg/L of nutrient medium was selected. Through the thin layer chromatography (in Sorbfill UV 254 plates) and column chromatography (Silica gel 60) there was evaluated the spectrum of shikonin derivatives in the course of culture establishment. Organogenic culture at the first steps of culturing on the LS-m nutrient medium was found to synthesize alongside with red fractions of shikonin pigmentes the blue ones as well. But after 1,5 year of cultivation Echium callus cells synthesized exclusively red fractions of shikonin pigments.

Key words: Echium plantagineum L., plant tissue culture, red pigments, shikonin derivatives, cell lines, producents of biologically active substances.

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ISSN 1995-5537

“Biotechnology” journal Vol. 1, No. 3, 2008
Р. 47-55, Bibliography 26, Ukrainian.
Universal Decimal classification 579.841:577.15

IMPROVEMENT OF BIOTECHNOLOGY

OF MICROBIAL EXOPOLYSACCHARIDE ETHAPOLAN ON ETHANOL

T. P. Pirog, Ju. V. Korzh

Zabolotny Institute of Microbiology and Virology of the National Academy of Science of Ukraine, Kiev

The study is devoted to investigating of the basic stages of С₂-compounds assimilation in Аcinetobacter sp. В-7005, to reveal «bottleneck» of С₂-metabolism and to develop of approaches to their removal to improve process of microbial exopolysaccharide ethapolan synthesis. Limitation of ethanol metabolism in Acinetobacter sp. В-7005 by the rate of acetate assimilation in a reaction catalyzed by acetyl-CoA-synthetase, was shown. Basing on the investigations of С₂-metabolism regulation the cultivation conditions were developed which allowed increasing the acetyl-CoA-synthetase activity in Acinetobacter sp. В-7005. Ethapolan synthesized under these conditions was characterized by high level of acylation (3?15%). Molecular mass of this polysaccharide was 1,5 million and was not changed during purification. Improved reological properties of ethapolan were associated with high molecular mass and increasing fatty acids content.

Key words: exopolysaccharides, biosynthesis, metabolism of ethanol, regulation of C₂-metabolism, physical and chemical properties.

Details: Hits: 25

ISSN 1995-5537

“Biotechnology” journal Vol. 1, No. 3, 2008
Р. 37-46, Bibliography 29, Ukrainian.
Universal Decimal classification 577.112.083:577.112.4:577.151.05

IMMUNOGLOBULINS OF COLOSTRUM

AS NOVEL MOLECULAR MARKERS OF PRECLINICAL DIAGNOSTICS OF AUTOIMMUNE DISORDERS IN PARTURIENT WOMEN

Yu. Ya. Kit, M. O. Starykovych, R. O. Bilyy, N. R. Skorohyd, L. B.Yanyv, R. S. Stoika

Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv
Lviv Regional Perinatal Center

Analysis of antigen specificity, catalytic and cytotoxic activity of colostrum immunoglobulins was carried out in order to detect novel molecular markers of autoimmune disorders. Immunoglobulins were isolated from parturient women colostrums by precipitation with 50% ammonium sulfate. It was shown that preparations of immunoglobulins possessed significantly different cytotoxic activity towards Jurkat leukemia T-cells, various hydrolyzing ability towards plasmid DNA and histone H1, and also differed by content of auto-antibodies to doublestranded DNA and histones. We concluded that functional properties of colostrums immunoglobulins depended on individual peculiarities of parturient women humoral immunity. These properties can be used for complex detection of early autoimmune disorders linked with pregnancy and delivery.

Key words: colostrum of healthy women, immunoglobulins, cytotoxic activity, catalytic activity, level of auto-AB.

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ISSN 1995-5537

“Biotechnology” journal Vol. 1, No. 3, 2008
Р. 25-36, Bibliography 107, Russian.
Universal Decimal classification 612.393:577.151.6

CATECHINS OF TEA: STRUCTURE, ACTIVITY, APPLICATION

V. A. Baraboy

In a review d technology of production of different kinds of tea and also the mechanisms of biological action of his the most active components — catechins are considered. An attempt to explain these mechanisms on the basis of antioxidant properties of these physiological active substances is undertaken, which are cause the presence of them antimutagen and antineoplastic effects. In detail chemical and biological properties of the most active from catechins — EGCG are considered.

Key words: tea, catechins, antioxidant activity.