BIOCOMPATIBILITY OF C60 FULLERENE WITH THE HEMOSTATIC SYSTEM V.O. Chernyshenko, O.P. Matyshevska, Y.D. Vinnіchuk, A.Yu. Labyntsev, O.E. Lugovska

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
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cover biotech acta general

Biotechnologia Acta V. 19, No. 3, 2026
P. 77-89, Bibliography 44 , Engl.
UDC: 577.3:615.28:612.12
doi: https://doi.org/10.15407/biotech19.03.077

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BIOCOMPATIBILITY OF C₆₀ FULLERENE WITH THE HEMOSTATIC SYSTEM

V.O. Chernyshenko, O.P. Matyshevska, Yu.D. Vinnіchuk, A.Yu. Labyntsev, O.E. Lugovska

Palladin Institute of Biochemistry of NAS of Ukraine, Kyiv, Ukraine

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Molecular compounds containing allotropic forms of carbon are intensively studied in various areas of science, such as nanobiotechnology, biomedicine, and pharmacology, for their practical application prospects. The most well-known carbon-based nanoparticles are the fullerene C₆₀, a spherical cage with distinctive physical, chemical, and biological characteristics (stability, biocompatibility, and antioxidant, antitumor, and photosensitizing properties). However, understanding the mechanisms by which these particles influence the body's regulatory and integral systems is necessary to prevent potential human health risks in clinical practice.
Aim. The impact of fullerene C₆₀ on the cardiovascular system was examined, with particular focus on hemostasis, to assess its safety for biomedical applications.

Methods. The study focused on the platelet, coagulation, anticoagulation, and fibrinolysis pathways of hemostasis.

Results. Research indicates that fullerene C₆₀ at therapeutic doses of 0.1 μM and 1.0 μM does not affect the functional activity, shape, or granularity of human platelets during co-cultivation. When present, measurements such as PTT, APTT, thrombin activity, factor Xa, plasma protein C, and the overall hemostatic potential of blood plasma remain stable.

Conclusions. Fullerene C₆₀ may be used in clinical settings as a bioinert carrier that interacts with blood at therapeutic doses without affecting or activating the hemostatic system.

Keywords: fullerene C₆₀, hemostasis, platelets, biocompatibility

Information about the authors
Volodymyr CHERNYSHENKO ORCID ID https://orcid.org/0000-0002-6564-8823
Olga MATYSHEVSKA ORCID ID https://orcid.org/0000-0003-0587-5124
Yulia VINNІCHUK ORCID ID https://orcid.org/0000-0002-3148-8067
Andrii LABYNTSEV ORCID ID https://orcid.org/0000-0002-1793-4630
Olga LUGOVSKA ORCID ID https://orcid.org/0000-002-1869-2503

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