USE OF PEA FRACTIONATION WASTES IN BIOETHANOL PRODUCTION E.A. Vovk, S.P. Tsygankov

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

cover biotech acta general

Biotechnologia Acta Т. 19, No. 1, 2026
P. , Bibliography , Engl.
UDC: 663.53
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USE OF PEA FRACTIONATION WASTES IN BIOETHANOL PRODUCTION

E.A. Vovk, S.P. Tsygankov

State Institution «Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine»

The production of protein and purified protein isolates from legumes using wet fractionation technology generates liquid starch-containing waste, a significant disposal problem. Such wastes can be considered feedstocks for biofuels, particularly bioethanol. In this way can be achieved a double effect: the valorisation of starch liquid wastes (SLW) and the partial replacement of food raw materials for energy needs.

Aim. To investigate the possibility of preparing a complex nutrient medium using standardised SLW after pea fractionation with the addition of milled corn for further fermentation to produce bioethanol.

Methods. Standardised SLW was obtained by the wet fractionation laboratory method; starch content was determined according to GSTU 46.045-2003, and protein content was determined by the Kjeldahl method in accordance with AOAC 979.09. Bioethanol was obtained in a laboratory fermenter using a simultaneous saccharification and fermentation (SSF) process. The leading indicators in the fermented samples were determined using the methods specified in SOU 15.9-37-242:2005.

Results. For all samples of combined wort with a concentration range of 25-29 % w/v dry matter (DM), the process can practically be completed in 72 hours. Еthanol content in the fermented wort was 10,6±0,05 – 12,1±0,05 % v/v for wort samples of the mentioned DM concentration.

Conclusions. During the fermentation of combined wort, pea starch, which is also present in the wort, gives an increase in the ethanol concentration in the fermented wort. The final ethanol yield from combined wort starch is low (0,6±0,04 l/kg), lower than from corn starch (0.665 l/kg). For the development of Very High Gravity (VHG) fermentation technology, it is necessary to select appropriate enzymes and parameters for the hydrofermentative preparation of combined wort.

Keywords: pea, starch liquid waste, fractionation, fermentation, enzymes, mash, VHG fermentation, bioethanol.