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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

cover biotech acta general

Biotechnologia Acta Т. 19, No. 1, 2026
P. 38-47, Bibliography  31, Engl.
UDC: 633.34:631.466:632.937
doi: https://doi.org/10.15407/biotech19.01.038

Full text: (PDF, in English)

PHYTOPATHOGENIC COMPONENT OF THE SOYBEAN ROOT MYCOBIOTA AND OPPORTUNITIES FOR THEIR BIOLOGICAL REGULATION

N. M. Serhiichuk1, 3, T. O. Rozhkova2., Y. V. Kolomiiets1

¹ National University of Life and Environmental Sciences of Ukraine, Kyiv
² Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
3 Open International University of Human Development “Ukraine”, Kyiv

Aim. To identify fungal phytopathogens from soybean roots and evaluate the antagonistic activity of Trichoderma spp. isolates alongside the antifungal efficacy of Streptomyces-based bioproducts against them.

Methods. Fungi were isolated on Czapek-Dox agar (HiMedia) and identified based on morphological and cultural features. The antagonistic potential of Trichoderma spp. was assessed via dual culture, while the antifungal efficacy of commercial bioproducts was determined using the agar diffusion method.

Results. Root mycobiota analysis revealed that Fusarium root rot is the dominant disease, caused by a complex of six species with F. solani prevailing (12.2–42.3%). In pesticide-free fields, natural regulation of F. solani by Trichoderma fungi was observed, whereas in commercial production, the presence of mycoparasites was negligible. Other pathogens (C. cassiicola, Ph. sojae, S. sclerotiorum) were harmful but localized. Efficient Trichoderma isolates and commercial bioproducts were screened for their ability to suppress pathogens.

Conclusions. Isolated Trichoderma strains exhibit significant antagonistic activity, particularly against F. oxysporum (70.9%). While Streptomyces-based bioproducts show limited antifungal efficacy against F. solani, they remain effective against other associated phytopathogens.

Keywords: phytopathogens, Trichoderma spp., Streptomyces spp., soybean root rot, Fusarium solani, microbial antagonism, biocontrol.

N. M. Serhiichuk1, 3 ORCID: 0000-0003-1624-1581 
T. O. Rozhkova² ORCID: 0000-0002-0791-9736 
Y. V. Kolomiiets1 ORCID: 0000-0002-1919-6336

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2026

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