ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 18, No. 2, 2025
P. , Bibliography 5 , Engl.
UDC 543.553+577.151
DEVELOPMENT AND ADAPTATION OF A BIOSENSOR BASED ON LACTATE OXIDASE AND POLY-M-PHENYLENEDIAMINE FOR THE DETERMINATION OF L-LACTATE IN REAL BIOLOGICAL SAMPLES
K.O. Berketa1,2, A.V. Buzhak1,2, Y.R. Vakhovksiy1,2, A.S. Sverstiuk3,4,
O.V. Soldatkina5, O.Y. Lyubovych6, S.V. Dzyadevych1,2, O.O. Soldatkin1,7
1Institute of Molecular Biology and Genetics he National Academy of Sciences of Ukraine, Kyiv
2Shevchenko National University of Kyiv, Ukraine
3Horbachevsky Ternopil National Medical University, Ukraine
4Ternopil National Ivan Puluj Technical University, Ukrainel
5Ternopil Regional Clinical Hospital, Ukraine
6Odesa Regional Pediatric Clinical Hospital, Ukraine
7National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
Aim. Development and optimization of an amperometric biosensor based on lactate oxidase with a poly-meta-phenylenediamine membrane for the accurate measurement of L-lactate in blood serum.
Material and Methods. The biosensor was based on a platinum disk amperometric transducer modified with a PPD membrane with immobilized LOx. Measurements were performed in a three-electrode amperometric system at a potential of +0.6 V. The actual sample results were verified spectrophotometrically and by an electrochemical analyzer.
Results. The biosensor demonstrated a wide linear range (7–1000 μM), a low detection limit (7 μM), and high selectivity. The standard addition method showed a high correlation (0.982) with reference methods.
Conclusions. The developed biosensor is promising for clinical diagnostics of blood lactate levels.
Keywords. Amperometric biosensor, lactate oxidase, real sample analysis, enzyme
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2025