ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta Т. 17, No. 2 , 2024
P. 24-26, Bibliography 4, Engl.
UDC:543.555; 577.15; 543.06
DOI: https://doi.org/10.15407/biotech17.02.024

Full text: (PDF, in English)

TECHNIQUE FOR IMPROVING THE ANALYTICAL CHARACTERISTICS OF BIOSENSORS BASEDON ENZYMES OF THE OXIDASE SUBCLASS

K.O. Berketa ^{1, 2}, D.O. Mruga ¹, S.V. Dzyadevych ^{1, 2}, O.O. Soldatkin ^{1, 3}

¹ Institute of Molecular Biology and Geneticsof the National Academy of Sciences of Ukraine, Kyiv
² Taras Shevchenko National University of Kyiv, Ukraine
3 National Technical University of Ukraine “Ihor Sikorsky Kyiv Polytechnic Institute”

Aim. In this study, the possibility of improving the analytical performance of a monoenzyme biosensor based on the main oxidase pathway by adding catalase to bioselective membranes was increased. Catalase helps strengthen the acid biomembrane, which facilitates the reaction that catalyzes glucose oxidase (GOD).

Methods. A standard glucose oxidase conductometric biosensor for increasing glucose was tested and a new bienzyme biosensor with glucose oxidase and catalase was created. To create bioselective membranes, enzymes were immobilized on the electrode surface for additional covalent formation of molecules in glutaraldehyde vapor.

Results. An optimal method for stabilizing enzymes and slowing down the biosensor operation depending on the catalase concentration was proposed. The stability of the biosensor preparation procedures and the recognition of the results were assessed. The presented analytical characteristics of a bienzyme biosensor with a monoenzyme based on GOD showed that the addition of catalase provided high efficiency, output of results and linear dynamism of the secret range operation.

Conclusion. The proposed method can be used to improve other oxidase-based biosensors.

Key words: conductometric converter, biosensor, oxidases, enzyme analysis.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024