ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 18-20, Bibliography 9, Engl.
UDC::543.556, 577.15, 621.314
DOI: https://doi.org/10.15407/biotech17.02.018
DEVELOPMENT OF CREATININE-SENSITIVE BIOSENSOR BASED ON IMMOBILIZED CREATININE DEIMINASE
V.A. Bakhmat 1, 2, O.O. Soldatkin 1, 3, V.M. Arkhypova 1, S.V Dzyadevych 1
1 Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv;
2 Taras Shevchenko National University of Kyiv, Ukraine
3 National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
Aim.Thе purpose of the work was to develop a new construction of enzyme biosensor based on creatinine deiminase for highly sensitive creatinine determination.
Methods. A new construction of enzyme biosensor based on creatinine deiminase was developed for the creatinine determination. A differential pair of gold interdigitated electrodes deposited onto a ceramic substrate was used as the electrochemical transducer. Creatinine deiminase was immobilized by cross-linking with glutaraldehyde on the surface of electrodes.
Results. The biosensor showed high sensitivity towards creatinine, the limit of detection was 5 µM. The biosensor was characterized by wide linear range of creatinine determination, high reproducibility of responses and showed high storage stability – after 50 days storage the biosensor retained 83% of the initial response value.
Conclusions. In future the developed biosensor can be used for express evaluation of the creatinine in biological samples.
Key words: biosensor, creatinine, creatinine deiminase, creatinine enzymatic assay.
References
1. Narayanan S., Appleton H. D. Creatinine: a review. Clinical chemistry, 1980, 26(8):1119‒1126. https://doi.org/10.1093/clinchem/26.8.1119
2. Kashani K., Rosner M.H., Ostermann M. Creatinine: from physiology to clinical application. European journal of internal medicine, 2020, 72: 9‒14. https://doi.org/10.1016/j.ejim.2019.10.025
3. Zakalskiy A., Stasyuk N., Gonchar M., Creatinine deiminase: characterization, using in enzymatic creatinine assay, and production of the enzyme. Curr. Protein Pept. Sci., 2019, 20(5): 465–470. https://doi.org/10.2174/1389203720666181114111731
4. Durán I., Espinosa-Mansilla A., Rodriguez Gómes M.J. Determination of methotrexate, several pteridines, and creatinine in human urine, previous oxidation with potassium permanganate, using HPLC with photometric and fluorimetric serial detection. Anal. Biochem., 2005, 346(2): 201‒209. https://doi.org/10.1016/j.ab.2005.07.038
5. Yokoyama Y., Tsuji S., Sato H. Simultaneous determination of creatinine, creatine, and UV-absorbing amino acids using dualmode gradient low-capacitycation-exchange chromatography. J. Chromatogr. A., 2005, 1085(1): 110‒116. https://doi.org/10.1016/j.chroma.2005.01.030
6. Zinellu A., Caria M.A., Tavera C., Sotgia S., Chessa R., Deiana, L., Carru C. Plasma creatinine and creatine quantification by capillary electrophoresis diode array detector. Anal. Biochem., 2005, 342(2): 186‒193. https://doi.org/10.1016/j.ab.2005.01.045
7. Marchenko S., Kucherenko I., Soldatkin O., Soldatkin, A. Potentiometric biosensor system based on recombinant urease and creatinine deiminase for urea and creatinine determination in blood dialysate and serum. Electroanalysis, 2015, 27(7): 1699‒1706. https://doi.org/10.1002/elan.201400664
8. Busono, P. (2015). Development of amperometric biosensor for creatinine detection. In 7th WACBE World Congress on Bioengineering 2015: 6th to 8th July, 2015, Singapore. Springer, Cham. 2015, 52: 134‒137. https://doi.org/10.1007/978-3-319-19452-3_36
9. Stasyuk N., Zakalskiy A.,. Nogala W, Gawinkowski S., Ratajczyk T., Bonarowska M., Demkiv O., Zakalska O., Gonchar M. A reagentless amperometric biosensor for creatinine assay based ons recombinant creatinine deiminase and N-methylhydantoin-sensitive CoCu nanocomposite. Sensors Actuators B, 2023, 393: 134276. https://doi.org/10.1016/j.snb.2023.134276dions}
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024