ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 2 , 2024
P. 18-20, Bibliography 9, Engl.
UDC::543.556, 577.15, 621.314
DOI: https://doi.org/10.15407/biotech17.02.018
DEVELOPMENT OF CREATININE-SENSITIVE BIOSENSOR BASED ON IMMOBILIZED CREATININE DEIMINASE
V.A. Bakhmat 1, 2, O.O. Soldatkin 1, 3, V.M. Arkhypova 1, S.V Dzyadevych 1
1 Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv;
2 Taras Shevchenko National University of Kyiv, Ukraine
3 National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
Aim.Thе purpose of the work was to develop a new construction of enzyme biosensor based on creatinine deiminase for highly sensitive creatinine determination.
Methods. A new construction of enzyme biosensor based on creatinine deiminase was developed for the creatinine determination. A differential pair of gold interdigitated electrodes deposited onto a ceramic substrate was used as the electrochemical transducer. Creatinine deiminase was immobilized by cross-linking with glutaraldehyde on the surface of electrodes.
Results. The biosensor showed high sensitivity towards creatinine, the limit of detection was 5 µM. The biosensor was characterized by wide linear range of creatinine determination, high reproducibility of responses and showed high storage stability – after 50 days storage the biosensor retained 83% of the initial response value.
Conclusions. In future the developed biosensor can be used for express evaluation of the creatinine in biological samples.
Key words: biosensor, creatinine, creatinine deiminase, creatinine enzymatic assay.