ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 16, No. 3, 2023
P. 45-50, Bibliography 15, Engl.
UDC: 582.31.99:574.1:57.085.2:615.322
DOI: https://doi.org/10.15407/biotech16.03.045
In vitro DIRECT SHOOT REGENERATION FROM Rhodiola rosea L. LEAF EXPLANTS
Matvieieva N., Belokurova V., Ratushniak Y., Shcherbak N.*, Kuchuk M.
Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv
Wild plant species are of great interest as a source of pharmacologically valuable compounds but a significant number of them are endemic and/or endangered ones. Modern plant biotechnology can provide reliable methods for their utilization without disturbing natural populations. In vitro culture methods for Rhodiola species are being intensively developed to include them in various biotechnological programs.
Aim. Development of a protocol for direct Rhodiola rosea L. plant regeneration from leaf explants.
Methods. The leaves of R. rosea aseptically growing plants were used as the explants. Several variants of Murashige and Skoog (1962) agar-solidified culture medium supplemented with different combinations of auxins (α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D)) and cytokinins (kinetin and 6-benzylaminopurine (BAP)) were estimated as potential regeneration-inducing media. Regeneration frequency was calculated as the percentage of leaves that produced shoots.
Results. The use of MS medium supplemented with 2.5 mg/l BAP and 1.0 mg/l 2,4-D allowed inducing shoot formation with 100% frequency. An increase in the 2,4-D content up to 2.5 mg/l and a decrease in BAP content to 1.0 mg/l resulted in decreasing in the regeneration frequency to 62.5%. Regeneration frequency was 25% and 62%, respectively, on the media containing 1.0 mg/l kinetin + 2.5 mg/l 2,4-D and 2.5 mg/l kinetin + 1.0 mg/l 2,4-D.
Conclusions. R. rosea leaf explants have demonstrated high regeneration capacity by using the studied combinations of plant growth regulators. MS medium supplemented with 2.5 mg/l BAP and 1.0 mg/l 2,4-D allowed for inducing shoot regeneration in leaf explants with a frequency of 100%. The frequency of regeneration was lower in the case of substitution of BAP for kinetin. The other types of morphogenesis (formation of adventitious roots and/or callus) were also observed.
Key words: Rhodiola rosea L., leaf explants, shoot regeneration, growth regulators.
Matvieieva N.
https://orcid.org/0000-0002-4877-5222
ResearcherID Web of Science: У-2237-2018
Belokurova V.
https://orcid.org/0000-0002-9872-9136
Web of Science ResearcherID: EMR-0642-2022
Ratushniak Y.
https://orcid.org/0000-0003-3708-1898
Web of Science ResearcherID: DNY-6665-2022
Shcherbak N.
https://orcid.org/0000-0002-2478-8408
Kuchuk M.
https://orcid.org/0000-0001-7365-7474
Web of Science ResearcherID: S-6631-2016
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2023
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