ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta V. 14, No 1, 2021
Р. 69-80, Bibliography 24, English
Universal Decimal Classification: 579.266
https://doi.org/10.15407/biotech14.01.069
QUANTITATIVE INDICATORS OF COPPER-RESISTANT MICROORGANISMS DISTRIBUTION IN NATURAL ECOSYSTEMS
O. А. Havryliuk1, V. М. Hovorukha1, А. V. Sachko2, G. V. Gladka2, О. B. Tashyrev1
1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2 Yuriy Fedkovych Chernivtsi National University, Ukraine
Copper is a highly toxic metal common in both natural and man-made ecosystems. The goal of the work was to determine the level of resistance of microorganisms of natural ecosystems to cationic form and organometallic complex of Cu2+. Microorganisms of 9 natural ecosystems of five geographic zones (the Antarctic, the Arctic, the Dead Sea (Israel), middle latitude (Ukraine) and the equatorial zone of South America (Ecuador) were investigated. Resistance of microorganisms was determined by cultivation in the medium with concentration gradient of Сu2+. The amount of Cu2+-resistant microorganisms in natural ecosystems was determined by colony counting on nutrient agar with Сu2+ citrate and Cu2+ cation. The Cu(II) concentration in soil and clay samples was analyzed by atomic absorption spectroscopy method. We have confirmed the hypothesis that microorganisms resistant to toxic Cu2+ compounds in high concentrations exist in any natural ecosystem. The resistance to Cu2+ cation was 8 – 31 and 14 –140 times less than to Cu2+ citrate in nutrient and mineral agar media respectively. The amount of Cu2+-resistant microorganisms in natural ecosystems reached hundreds and thousands at the presence of 175…15 500 ppm Cu2+. Thus, the soils, clays and sands of natural ecosystems are a “genetic resource” of copper-resistant microorganisms that are promising for development of novel biotechnology of purification of copper-containing wastewater and soil bioremediation.
Key words: copper pollution, copper-resistant microorganisms, natral ecosystems, environmental biotechnologies.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2021
References
1. Flemming C. A., Tr evors J. T. Copper toxicity and chemistry in the environment: a review. Water, Air, & Soil Pollution. 1989, 44 (1– 2), 143–158. https://doi.org/10.1007/BF00228784
2. Schouten C. The role of sulphur bacteria in the formation of the so-called sedimentary copper ores and pyritic ore bodies. Economic Geology. 1946, 41 (5), 517–538.
3. Ashida J., Higashi N., Kikuchi T. An electronmicroscopic study on copper precipitation by copper-resistant yeast cells. Protoplasma. 1963, 57 (1–4), 27–32. https://doi.org/10.1007/BF01252044
4. Dovgalyuk А. Environmental pollution by toxic metals and its indication by plant test systems. Studia Biologica. 2013, 7 (1), 197–204. https://doi.org/10.30970/sbi.0701.269
5. Masindi V., Muedi K. L. Environmental contamination by heavy metals. Heavy Metals. 2018, P. 115–133.https://doi.org/10.5772/intechopen.76082
6. Husak V. Copper and copper-containing pesticides: metabolism, toxicity and oxidative Stress. J. Vasyl Stefanyk Precarpathian National University. 2015, 2 (1), 38–50. https://doi.org/10.15330/jpnu.2.1.38-50
7. Andreazza R., Pieniz S., Okeke B. C., Camargo F. A. O. Evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption. Brazil. J. Microbiol. 2011, 42 (1), 66–74. https://doi.org/10.1590/S1517-83822011000100009
8. Hovorukha V., Havryliuk O., Tashyreva H., Tashyrev O., Sioma I. Thermodynamic substantiation of integral mechanisms of microbial interaction with metals. Ecological Engineering and Environment Protection. 2018, V. 2, P. 55–63, https://doi.org/10.32006/eeep.2018.2.5563.
9. Kibria G. Trace/heavy metals and its impact on the environment, biodiversity and human health- a short review. Proj. Rech. 2016, P. 1–5. https://doi.org/10.13140/ RG.2.1.3102.2568
10. Stenberg B., Johansson M., Pell M., Sj?dahlSvensson K., Stenstr?m J., Torstensson L. Microbial biomass and activities in soil as affected by frozen and cold storage. Soil Biol. Biochem. 1998, 30 (3), 393–402. https://doi.org/10.1016/S0038-0717(97)00125-9
11. Baker T. H. W. Transportation, preparation, and storage of frozen soil samples for laboratory testing. Soil Specimen Preparation for Laboratory Testing. D. A. Sangrey, R. J. Mitchell, Eds. West Conshohocken, PA: ASTM International. 1976, P. 88–112.
12. Public Health England. Detection and enumeration of bacteria in swabs and other environmental samples. National Infection Service Food Water and Environmental Microbiology Standard Method. 2017, 4 (4).
13. Ogunfowokan A. O., Adekunle A. S., Oyebode B. A., Oyekunle J. A. O., Komolafe A. O., Omoniyi-Esan G. O. Determination of heavy metals in urine of patients and tissue of corpses by atomic absorption apectroscopy. Chemistry Africa. 2019, 2 (4), 699–712. https://doi.org/10.1007/s42250-019-00073-y
14. Prekrasna I. P., Tashyrev O. B. Copper resistant strain Candida tropicalis RomCu5 interaction with soluble and insoluble copper compounds. Biotechnol. acta. 2015, 8 (5), 93–102. https://doi.org/10.15407/biotech8.05.093
15. Samanovic M. I., Ding C., Thiele D. J., Darwin K. H. Copper in microbial pathogenesis: meddling with the metal. Cell Host Microbe. 2012, 11 (2), 106–115, https://doi.org/10.1016/j.chom.2012.01.009
16. Borkow G., Gabbay J. Copper as a biocidal tool. Current Medicinal Chemistry. 2005, 12 (18), 2163–2175.https://doi.org/10.2174/0929867054637617
17. Havryliuk O., Hovorukha V., Tashyrev O. The resistance of chernozem soil microorganisms to soluble copper compounds. Factors in Experimental Evolution of Organisms. 2018, V. 3826, P. 273–278. (In Ukrainian).
18. Kisel V. I. Soil pollution by heavy metals. Agroecological assessment of Ukrainian lands and placement of agricultural crops. Kyiv: Agricultural Science. 1997, 160 p. (In Russian).
19. Mineev V. G. Chemicalization of agriculture and natural environment. Moscow: Agropromizdat. 1990. (In Russian).
20. Ochoa-Herrera V., Le?n G., Banihani Q., Field J. A., Sierra-Alvarez R. Toxicity of copper(II) ions to microorganisms in biological wastewater treatment systems. Science of the Total Environment. 2011, V. 412–413, P. 380–385. https://doi. org/10.1016/j.scitotenv.2011.09.072
21. Rajbanshi A. Study on heavy metal resistant bacteria in Guheswori Sewage Treatment Plant. Our Nature. 2009, V. 6. https://doi. org/10.3126/on.v6i1.1655
22. Parungao M. Biosorption of copper, cadmium and lead by copper-resistant bacteria isolated from Mogpog River, Marinduque. Philippine J. Sci. 2007, 136 (2), 155–165.
23. Brahmaprakash G. P., Deva sia P., Jagadish K. S., Na tara jan K. A., Ramananda Rao G. Development of Thiobacillus ferrooxidans ATCC 19859 strains tolerant to copper and zinc. Bulletin of Materials Science. 1988, 10 (5), 461–465. https://doi.org/10.1007/BF02744659
24. Havryliuk O., Hovorukha V., Patrauchan M., Youssef N. H., Tashyrev O. Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4. Current Research in Microbial Sciences. 2020, V. 1, P. 44–52. https://doi.org/10.1016/j.crmicr.2020.06.002