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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)


Biotechnologia Acta V. 13, No 6, 2020
Р. 41-49, Bibliography 34, English
Universal Decimal Classification: 616.11


 A. A. Sokol, D. A. Grekov, G. I. Yemets, N. V. Shchotkina, A. A. Dovghaliuk, N. M. Rudenko, I. M. Yemets

State Institution "Scientific - Practical Medical Center of Pediatric Cardiology and Cardiac Surgery of the Ministry of Health of Ukraine", Kyiv

The aim of the study was to estimate the properties of the scaffold obtained by decellularization of bovine pericardium with a 0.1% solution of sodium dodecyl sulfate. The experiment included standard histological, microscopic, molecular genetic, and biomechanical methods. Scaffold was tested in vitro for cytotoxicity and in vivo for biocompatibility. A high degree of removal of cells and their components from bovine pericardium-derived matrix was shown. Biomechanical characteristics of artificial scaffold were the same as those of the native pericardium. With prolonged contact, no cytotoxic effect on human cells was observed. The biointegration of the scaffold in laboratory animals tissues was noted, which confirms the potential possibility of the implant applicationin cardiac surgery.

Key words:. scaffold, decellularization, cardioimplant, tissue engineering.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2020