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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)


Biotechnologia Acta V. 13, No 4, 2020
Р. 49-59 , Bibliography 31, English
Universal Decimal Classification:  579.695


V .M. Hovorukha, O. A. Havryliuk, G. V. Gladka, O. B. Tashyrev

Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv,

Thermodynamic calculations allow determining optimal metabolic pathways for microbial extraction of toxic soluble hexavalent chromium compounds from contaminated sewage.

The purpose was to predict theoretically and confirm experimentally the possibility of hazardous Cr(VI) removal by hydrogen producing microbiome with simultaneous destruction of multicomponent organic waste and hydrogen synthesis.

The gas composition was determined by the standard gas chromatography method. The redox potential (Eh) and рН of the medium were measured potentiometrically. The Cr(VI) concentration was measured by a photocolorimetric method.

The multicomponent organic waste was effectively destroyed by hydrogen producing microbiome at the absence of chromium. The hydrogen fermentation cycle was not significantly inhibited by addition of Cr(VI). After complete microbial reduction of soluble CrO42– to insoluble Cr(OH)3·nH2O↓ the metabolic parameters returned to initial values.

The optimal pathway of microbial detoxification of toxic Cr(VI) compounds was thermodynamically predicted and experimentally confirmed. The high efficiency of Cr(VI) removal by strict anaerobic hydrogen producing microbiome via dark hydrogen fermentation of multicomponent organic waste was demonstrated. The obtained results can be used for development of novel environmental biotechnology of chromium-containing sewage purification and simultaneous destruction of environmentally hazardous organic waste as well as obtaining of eco-friendly energy carrier biohydrogen.

Key words: thermodynamic prediction, environmental biotechnologies, hydrogen fermentation, biohydrogen synthesis, toxic chromium(VI) compounds, microbial reduction of chromate, multicomponent organic waste destruction.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2020