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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

2 2018

"Biotechnologia Acta" V. 11, No 2, 2018
Р. 72-78, Bibliography 23, English
Universal Decimal Classification:  57.017.22


L. N. Churkina 1, V. V. Klochko 1, S. .D Zagorodnya 1, L. V. Yaroshenko 1, O. B. Luitko 2

1Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2Institute of Orthopedics and Traumatology of the National Academy of Medical Sciences of Ukraine, Kyiv

The aim of the work was the research of antibiotic batumin action on the biofilm formed by staphylococci and on the process of the biofilm formation by strain-producer Pseudomonas batumici. The minimal inhibiting concentration (MIC) of batumin was studied according to The Clinical and Laboratory Standards Institute standards — CLSI. Biofilm formation was studied using photometric O’Toole method with flatbed photometer at wavelength 540 nm. The batumin penetrated into biofilms of staphylococci and reduced the biomass of the formed biofilm of Staphylococcus aureus and S. epidermidis at the antibiotic concentration of 0.5 ?g/ml, which was only twice higher than MIC of batumin for planktonic cells. The degree of biofilm changes in the presence of batumin differed for different staphylococcus strains. However, these differences did not depend on strains sensitivity or their resistance to the antibiotic.

The ability of batumin strain-producer to form the biofilm and the ability of antibiotic to stimulate this process in concentrations of 1 and 10 ?g/ml was established for the first time. In addition, it was found that in the Pseudomonas batumici B-321 planktonic culture in the presence of 10 ?g/ml of batumin, the increase in length of producer cells on average by 15% was noted.

The obtained data shed the light on batumin antimicrobic action on the staphylococci biofilm. It can be argued that is a perspective for treatment of staphylococcal infections. It enabled to consider batumin as a promising drug for the staphylococcal infections treatment.

Key words: batumin, staphylococci, biofilm.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018