ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 8, No 5, 2015
https://doi.org/10.15407/biotech8.05.086
Р. 86- 92, Bibliography 15, English
Universal Decimal Classification: 579:662.7
SPECIFIC FEATURES OF NATIVE CHEMOLITHOTROPHIC MICROBIOTA WASTES PRODUCED BY ENERGY INDUSTRY
I. A. Blayda, T. V. Vasilyeva, L. I. Slyusarenko, V. F. Khytrych
Odessa I. I. Mechnikov National University, Ukraine
The study's aims were to isolate and establish pure cultures of prevailing microorganisms from the aboriginal consortium in coal fly ash (FAAC), describe their physiology, biochemistry and practically-useful properties, and compare the efficiency of bioleaching metals from fly ash using pure cultures and the consortium. Through enrichment cultures on standard media we isolated pure cultures of the microorganisms which were then preliminarily identified using standard techniques. This allowed us to isolate from FAAC pure cultures of three prevailing strains of mesophilic and moderately thermophilic acidophilic chemolithotrophic bacteriae, belonging to Acidithiobacillus, such as Acidithiobacillus ferrooxidans, and Sulfobacillus. The strains exhibited high oxidative activity in leaching the rare metals Gallium and Germanium, as well as some heavy metals, from fly ash substrate. A comparison of oxidative activity of the isolated strains and the aboriginal consortium under mesophilic conditions led to the conclusion about advantage of consortium, because it had arisen from syntrophy of microbes in the community. This should be taken into account at the developing of bacterial preparations that are optimal for the technogenic substrate.
Key words: fly ash, aboriginal microbial consortia, acidophilic chemolithotrophic bacteriae, leaching activity, Germanium.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2015
References
1. Karavayko G. I., Kuznetsov S. I., Golomzik Ye. I. The Role of Microorganisms in the leaching of metals from ores. Moskva: Nauka. 1972, 248 p. (In Russian).
2. Brierley J. A. Expanding role of microbiology in metallurgical processes. Mining Engin. 2000, 52 (11), 49–53.
3. Vasil'eva T. V., Blayda I. A., Ivanitsa V. A. Тhe main groups of microorganisms involved in the biohydrometallurgical process. Problemy ekolohіchnoy bіotekhnologіi. 2013, 1. Available at: http://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/4678. (In Russian).
4. Ivanov M. V., Karavayko G. I. Geological microbiology. Mikrobiologiya. 2004, 5 (73), 581–597. (In Russian).
5. Norris P. R., Burton N. P., Foulis N. A. M. Acidophiles in bioreactor mineral processing. Extremophiles. 2000, V. 4, P. 71–76.
6. Blayda I. A. Extraction of valuable metals from industrial waste biotechnological methods (Review). Energotekhnologii i resursosberezhenie. 2010, V. 6, P. 39–45. (In Russian).
7. Blayda I. A., Vasileva T. V., Slyusarenko L. I., Barba I. N., Ivanitsa V. A. Composition and leaching activity of energy industrial waste microbiocenosis. Problemy ekologіchnoy bіotekhnologіi. 2013, 1. Available at: http://jrnl.nau.edu.ua/index.php/ ecobiotech/article/view/4592. (In Russian).
8. Kuzyakina T. I., Haynasova T. S., Levenets O. O. Biotechnology extraction of metals from sulfide ores. Vestnik nauk o Zemle. 2008, 60 (12), 76–85. (In Russian).
9. Blayda I. A., Vasileva T. V., Slyusarenko L. I., Khitrich V. F., Ivanitsa V. A. Еxtraction of rare and nonferrous metals by microbial communities of the ash from burning Рavlograd’s coal. Microbiology & Biotechnology. 2012, V. 3, P. 91–101. (In Russian).
10. Kondrateva T. F., Pivovarova T. A., Tsaplina I. A. A variety of community chemolithotrophic acidophilus microorganisms in natural and man-made ecosystems. Mikrobiologiya. 2012, 1 (81), 3–27. (In Russian).
11. Karavayko G. I. Practical Guide to biogeotechnology metals. Moskva: AN SSSR. 1989, 371 p. (In Russian).
12. Methods for General Bacteriology. V. 2. Moskva: Mir. 1984, 265 p. (In Russian).
13. Chernyak S. M., Kolobova T. P., Pershina I. V. Methods of hydro-chemical analysis of objects of the marine environment. In: Methodical bases of an integrated environmental monitoring of ocean. Мoskva: Gidrometeoizdat. 1988, P. 23–25. (In Russian).
14. Khavezov I., Tsalev D. Atomic absorption analysis. Leningrad: Khimiya. 1983, 144 p. (In Russian).
15. Kellу D. P., Wood A. P. Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Hallothiobacillus gen. nov. and Thermithiobacillus gen. nov. Int. J. Syst. Evolution. Microbiol. 2000, V. 50, P. 512–516.