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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

 6 2014

"Biotechnologia Acta" v. 7, no 6, 2014
https://doi.org/10.15407/biotech7.06.057
Р. 57-62, Bibliography 16, Russion
Universal Decimal classification: 615.281-546.57-541.8-576.8.06

OBTAINING AND ACTIVITY OF SILVER NANOPARTICLES BASED ON THE EXOPOLYSACCHARIDE OF DIAZOTROPHIC STRAIN Bradyrhizobium japonicum 36 AND AgNO3

B. A. Rasulov

Institute of Genetics and Plant Experimental Biology of the Uzbekistan Academy of Sciences Tashkent Province

The aim of the research was to obtain silver nanoparticles and to study of its antibacterial activity against pathogenic cultures Escherichia coli ATCC11229 and Staphylococcus  aureus ATCC6538.

Obtained complex was characterized by UV-Vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). Colloid solutions  of  the  complex  absorbed  radiation in  the  visible  regions  of  420–460  nm,  which confirmed the formation of silver nanoparticles. The  size  of  synthesized  SNP  varied  from  6  to  50  nm.  There has been confirmed presence in them of atomic silver.  Obtained compound possessed antibacterial activity against pathogens, such as Escherichia coli ATCC11229 and Staphylococcus aureus ATCC6538.

Key words: silver  nanoparticles,  Bradyrhizobium japonicum 36 strain exopolysaccharide.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014