ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 6, 2014
https://doi.org/10.15407/biotech7.06.046
Р. 46-50, Bibliography 14, English
Universal Decimal classification: 577.181.4
BIOSYNTHESIS AND PROPERTIES OF ANTIBIOTIC BATUMIN
Istitute of Micribiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
Biosynthesis of antistaphylococcal antibiotic batumin under periodic conditions of Pseudomonas batumici growth has been studied. Antibiotic synthesis in fermenter occurred across the culture growth and achieved its maximal value after 50–55 hours. The active oxygen utilization by the producing strain was observed during 20–55 hours of fermentation with maximum after 40–45 hours. Antibiotic yield was 175–180 mg/l and depended on intensity of aeration.
contrast to "freshly isolated" antibiotic after fermentation the long-term kept batumin has shown two identical by molecular mass peaks according to the chromato-mass spectrometric analysis. Taking into account of batumin molecule structure the conclusion has been made that the most probable isomerization type is keto-enolic tautomerism. At the same time batumin is diastereoisomer of kalimantacin A which has the same chemical structure. The optic rotation angle is [α]d25 = +56.3° for kalimantacin and [α]d25 = –13.5° for batumin.
The simultaneous P. batumici growth and antibiotic biosynthesis and the ability of this molecule to optical isomerisation and keto-enolic forms formation allow us to suppose that batumin plays a certain role in metabolism of the producing strain.
References
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
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1. Bernd H., Rehm A. Pseudomonas: Model Organism, Pathogen, Cell Factory. Verlag GmbH.: Wiley-VCH. 2008, 403 p.
2. El-Sayed A. K., Hothersall J., Cooper S. M., Stephens E., Simpson T. J., Thomas C. M. Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586. Chem. Biol. 2003, N 10, P. 419–430.
http://dx.doi.org/10.1016/S1074-5521(03)00091-7
3. Klochko V. V., Kiprianova E. A., Churkina L. N., Avdeeva L. V. Antimicrobial spectrum of antibiotic batumin. Microbiol. zh. 2008, 70 (5), 41–46. (In Russian).
4. Kiprianova E. A., Klochko V. V., Zelena L. B., Churkina L. N., Avdeeva L. V. Pseudomonas batumici sp. nov., the antibiotic-producing bacteria isolated from soil of the Caucasus Black Sea coast. Microbiol. zh. 2011, 73 (5), 3–8. (In Russian).
5. Mir-Kadyrova E. Y. Structure of antibiotic batumin. M. S. thesis. Moskva, 1982. (In Russian).
6. Churkina L., Kiprianova E., Bidnenko S., Marchenko K. Antibiotic batumin for diagnostics of Staphylococci and treatment of Staphylococcus aureus nasal carriage. Likarska sprava. 2009, N 1–2, P. 61–67. (In Ukrainian).
7. Klochko V. V. Significance of mineral nutrition at the process of antistaphylococcal antibiotic batumin biosynthesis. Naukovyi visnyk Chernivetskoho univer. 2004, N 194, P. 17–22. (In Ukrainian).
8. Klochko V. V., Kiprianova E. A. Physical-chemical and microbiological methods of assay of antistaphylococcal antibiotic batumin. Naukovyi visnyk Chernivetskoho univer. 2006, N 197, P. 62–66. (In Ukrainian).
9. Moloney M. Structure and Reactivity in Organic Chemistry. Wiley-Blackwell, 2008, 306 p.
10. Lykov V. P., Bodnar I. V., Hovrychev M. P. Limitation of grows and biosynthesis of gramicidin C of Bacillus brevis var. G.-B. Mikrobiologiia. 1985, 54 (2), 245–251. (In Russian).
11. Matselyukh B. P., Tymchyk O. V., Matselyuh A. B. Influence of Streptomyces globisporus 3-1 growth on the synthesis landomicin E. Microbiol. zh. 2005, 67 (4), 44–51. (In Russian).
12. Kiviharju K., Leisola M., Eerikainen T. Optimization of Streptomyces peucetius var. caesius N 47 cultivation and erhodomycinone production using experimental designs and response surface methods. J. Ind. Microbiol. Biotechnol. 2004, N 31, P. 475–481.
http://dx.doi.org/10.1007/s10295-004-0172-3
13. Carey F., Sundberg R. Advanced Organic Chemistry. Springer, 2007. 1199 p.
14. Tokunaga T., Kamigiri K., Orita M. Kalimantacins A, B and C, novel antibiotics from Alcaligenes sp. YL-02632S. II. Physico-chemical properties and structure elucidation. J. Antibiot. 1996, 49 (2), 140–144.
http://dx.doi.org/10.7164/antibiotics.49.140