ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 4, 2014
https://doi.org/10.15407/biotech7.04.049
Р. 49-53, Bibliography 17, English
Universal Decimal classification: 663.12: 575.224.4: 577.161.1
ISOLATION OF Phaffia rhodozyma YEASTS MUTANTS UNDER INCREASED CAROTENOID CONTENT
Matselyukh B. P. 1, Matselyukh D. Ya. 1, Golembiovska S. L. 1, Gural S. V. 2
1Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2Institute of Animal Biology of the National Academy of Agrarian Sciences of Ukraine, Lviv
The aim of the paper was the selection of the improved strains of Phaffia rhodozyma using chemical mutagenesis and the identification of individual carotenoids synthesized by isolated more pigmented mutants. Hyperpigmented mutants of P. rhodozyma NRRL Y-17268-1 and IMB Y-5021-15 were isolated from initial strains NRRL Y-17268 and IMB Y-5021 by nitrosoguanidine mutagenesis. Pigments were purified by TLC and identified using HPLC and liquid chromatography-mass spectrometry. It was shown that initial strains P. rhodozyma NRRL Y-17268 and IMB Y-5021 and obtained from them mutants NRRL Y-17268-1 and IMB Y-5021-15 produced torulene and torularhodin without illumination in shaking flasks at 20 оC. The content of torularhodin produced by the mutant strains Y-17268-1 (18.2 ?g) and Y-5021-15 (16.5 ?g) per 1.0 g of dry biomass was increased to 33.8 and 18.4%, respectively, in comparison with the content of this pigment in the initial parental strains. The obtained strains present interest for further selection of more active producers of carotenoids and examination of the action of reactive oxygen species as stimulators of carotenoid production in yeasts.
Key words: Phaffia rhodozyma, mutagenesis, torulene, torularhodin.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008
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