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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

3 2014

"Biotechnologia Acta" v. 7, no 3, 2014
https://doi.org/10.15407/biotech7.03.074
Р. 74-80, Bibliography 10, Russian
Universal Decimal classification: 544.7+577.1:546.284-31:543.42

PECULIARITIES OF WATER FREEZING IN CRYOPROTECTIVE MEDIUM IMPLEMENTED IN A MATRIX OF HYDROPHOBIC SILICA BULL SPERM

V. V. Turov, T. V. Krupskaya, N. P. Galagan

Chuiko Institute of Surface Chemistry of the National Academy of Sciences of Ukraine, Kyiv

The study of the process of melting water in lactose-glycerol-yolk kriomedium containing gametes bull, incorporated in the hydrophobic silica powder, which are adsorbed on the surface of fixed amounts of nonpolar hydrocarbon – n-decane was the aim of the work. The possibility of water polyassociates structuring with a solid surface of interfacial water and solubility of trifluoroethanoic acid in it have been studied. Thereat survival of the germ cell after contact with the surface was not analyzed.

State of water in initial cryoprotective glycerol-lactose-yolk medium and hydrophobic nanosilica TS-100 containing n-decane additive adsorbed on its surface incorporated in a matrix was studied using low-temperature 1H-NMR spectroscopy method. It is shown that the solid matrix induces formation of 6–7 water molecules per each dean molecule at the interface, which do not take part in formation of hydrogen bonds, and a sharp radius decrease (from 100 to 20 nm) of ice crystals formed in cell suspension at its freezing. The results could give rise to safety improving of their cells at their cryopreservation and low temperature storage conditions by incorporating into a powder composite environment.

Key words: 1H-NMR spectroscopy, bull gametes, strongly and weakly associated water, water clusters.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014

References

1.  Hubalek Z. Protectants used in the cryopreservation of microorganisms. Cryobiology. 2003, V. 46, P. 205–229.
https://doi.org/10.1016/S0011-2240(03)00046-4

2.  Karlson J. O. M., Toner M. Long­term storage by cryopreservation: critical issues. Biomaterials. 1996, V. 17, P. 243–256.
https://doi.org/10.1016/0142-9612(96)85562-1

3.  Cucaeva А. А. (Ed). Crioconservation of cells suspensions. Kyiv: Naukova dumka. 1983, 240 p. (In Russian).

4.  Pushkar N. C. (Ed). Cryoconservation of cells and tissues. Kharkіv Institute of problems of cryobiology and cryomedicine. 1989, 187 p. (In Russian).

5.  Gun’ko V. M., Turov V. V., Gorbik P. P. Water on interfaces. Kyiv: Naukova dumka. 2009, 694 p. (In Russian).

6.  Turov V. V., Gun’ko V. M. The clustered water and ways of its applications. Kyiv: Naukova dumka. 2011, 313 p. (In Russian).

7.  Gun’ko V. M., Turov V. V. Nuclear Magnetic Resonance Studies of Interfacial Phenomena. New York: Taylor & Francis. 2013, 1076 p.
https://doi.org/10.1201/b14202

8.  Petrov O. V., Furo I. NMR cryoporometry: Principles, application and potential. Progr. NMR. 2009 V. 54, P. 97–122.
https://doi.org/10.1016/j.pnmrs.2008.06.001

9.  Turov V. V., Kerus S. V., Gunko V. M. Behavior of water bound in bone marrow cells affected by organic solvents of different polarity. Cryobiology. 2009, V. 59, P. 102–112.

10.  Pople J. A., Schneider W. G., Bernstein H. J. High­Resolution Nuclear Magnetic Resonance. New York: McGraw­Hill Book Company. 1959, Р. 165–180.