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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

2 2014

 "Biotechnologia Acta" v. 7, no 2, 2014
https://doi.org/10.15407/biotech7.02.034
Р. 34-45, Bibliography 46, English.
Universal Decimal classification: 577.112: 543.544.17(045)

APPLICATION OF IMMUNOGLOBULIN-BINDING PROTEINS A, G, L IN THE AFFINITY CHROMATOGRAPHY

О. V. Sviatenko1, O. B. Gorbatiuk2, О. А. Vasylchenko1

1National Aviation University, Kyiv, Ukraine
2State Institute of Genetic and Regenerative Medicine of National Academy of Medical Sciences  of Ukraine, Kyiv

Proteins A, G and L are native or recombinant proteins of microbial origin that bind to mammalian immunoglobulins. Preferably recombinant variants of proteins A, G, L are used in biotechnology for affinity sorbents production. Сomparative characteristics of proteins A, G, L and affinity sorbents on the basis of them, advantages and disadvantages of these proteins application as ligands in the affinity chromatography are done. Analysis of proteins A, G, L properties is presented. Binding specificities and affinities of these proteins differ between species and antibody subclass. Protein А has high affinity to human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, IgG3, goat and sheep IgG2, dog, cat, guinea pig, rabbit IgG. Protein G binds strongly to human, mouse, cow, goat, sheep and rabbit IgG. Protein L has ability of strong binding to immunoglobulin kappa-chains of human, mouse, rat and pig. Expediency of application of affinity chromatography with usage of sorbents on the basis of immobilized proteins A, G, L are shown for isolation and purification of antibodies different classes. Previously mentioned method is used as an alternative to conventional methods of protein purification, such as ion-exchange, hydrophobic interactions, metal affinity chromatography, ethanol precipitation due to simplicity in usage, possibility of one-step purification process, obtaining of proteins high level purity, multiuse at maintenance of proper storage and usage conditions. Affinity sorbents on the basis of immobilized proteins A, G, L are used not only for antibodies purification, but also for extraction of different antibodies fractions from blood serum.

Key words: affinity chromatography, Staphylococcus protein А, peptostreptococcal protein L, protein G.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014

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