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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

 6 2013

"Biotechnologia Acta" v. 6, no 6, 2013
Р. 105-112, Bibliography 12, Ukrainian.
Universal Decimal classification: 579.222


A. F. Tkachenko, N. E. Beyko, A. I. Khomenko, O. O. Tigunova, S. E. Priyomov, S. M. Shulga

SO «Institute of Food Biotechnology and Genomics» of National Academy of Sciences of Ukraine, Kyiv

Scientific research of essential amino acids biotechnology is directed both to create optimum conditions for producer’s cultivation and economically viable raw materials selection for these technologies, so as breeding the more productive microorganisms strains capable of extracellular producing amino acids.

For successful microbial synthesis it is necessary to have an excellent crop’s metabolism knowledge and ensure that the composition of growth medium have no repressing substances.

Bacterial cultures from «Collection microorganism’s stains and plants line for food and agriculture biotechnology» from Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine have been studied. Tryptophan producer Bacillus subtilis have been selected, which accumulated the greatest amount of this amino acid in the cultivation liquid. The optimal culture producer conditions were selected. Using selection methods, namely mutagenesis with UV irradiation and sequential stepwise selection, mutant strain Bacillus subtilis IFBG MC-1 were obtained which produced nearly 50% more tryptophan (13.9 g/l) than the parent strain.

Key words: Bacillus subtilis, biosynthesis, tryptophan, stain.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013


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