ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)
"Biotechnologia Acta" v. 6, no 6, 2013
https://doi.org/10.15407/biotech6.06.094
Р. 94-99, Bibliography 23, Ukrainian.
Universal Decimal classification: 579.841.222
GRAMINEAE PLANTS GROWTH STIMULATION BY SURFACE-ACTIVE RHAMNOLIPIDS
O. V. Karpenko1, N. I. Koretska1, N. S. Shcheglova1, I. V. Karpenko1, V. I. Baranov2
1Litvinenko Іnstitut of Physical and Organic Chemistry and Coal Fuel Chemistry of National Academy of Sciences of Ukraine
2Franko Lviv National University, Ukraine
The effect of biogenic surfactants which were synthesized by the culture Pseudomonas sp. PS-17, particularly rhamnolipid biocomplex, on the growth of cereal plants — wheat and barley in the early stages of vegetation, as well as its effect on the activity of indole-3-acetic acid was studied. The effect of rhamnolipid biocomplex on the growth activity of winter wheat and spring barley was studied in vegetation (sand culture) and field experiments with pre-sowing treatment of seeds with rhamnolipid biocomplex solutions of different concentrations. To investigate the action of surfaceactive rhamnolipid biocomplex on the extension growth of plant cells the specific biotest on sections of wheat coleoptiles was used.
The stimulating effect of rhamnolipid biocomplex on the growth of cereal plants, as well as the optimal concentration for its use in pre-sowing treatment of seeds was established: for winter wheat — 10 mg/l, for spring barley — 50 mg/l (Р ≤ 0,05). The application of rhamnolipid biocomplex contributed to the increase of aboveground dry weight of wheat on 16%, of barley — on 18% compared to the control (Р ≤ 0,05). Rhamnolipid biocomplex effectiveness was also confirmed in the field experiment — after the treatment of spring barley seeds the vegetative mass was averagely 34% (Р ≤ 0,05) higher than in the control.
The obtained results indicate the prospects of practical use of rhamnolipid surfactants as environmentally friendly growth stimulants for cereal plants for modern technologies in agriculture. They can be effective in creating complex preparations with indole-3-acetic acid and, possibly also with other phytohormones, that will allow the enhancement of their activity.
Key words: Gramineae plants, stimulation of plant growth, biogenic surfactants, rhamnolipids, indole-3-acetic acid.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013
References
1. Zlotnikov A. K., Dyerov A. I., Begunov I. I., Zlotnikov K. M. Album on winter wheat. Zemledelie. 2005, N 3, P. 31–32. (In Russian).
2. Bioregulation of microbial-plant systems. Ed. G. A. Iutinskoy, S.?P. Ponomarenko. Кyiv: Nichlava. 2010, 463 p. (In Russian).
3. Wally O. S. D., Critchley A. T., Hiltz D. Regulation of Phytohormone Biosynthesis and Accumulation in Arabidopsis Following Treatment with Commercial Extract from the Marine Macroalga Ascophyllum nodosum. J. Plant Growth Regul. 2013, 32 (2), Р. 324.
4. Ulyanenko L. N., Arysheva S. P., Fillipas?A. S. Influence of zircon on growth of wheat and accumulation of Cd in crops. S.-kh. biol. 2005, N 1, P. 39–43. (In Russian).
5. Tyuterev S. L. Scientific bases of induced disease resistance of plants. SPb.: RASKHN VNIIZR. 2002, 328 p. (In Russian).
6. Muthusamy K., Gopalakrishnan S. R. Biosurfactants: properties, commercial production and application. Curr. Sci. 2008, V. 94, P. 736–747.
7. Pat. Ukrainy N 71792 А,15. МПК С12N 1/02, C12R 1:38. Surface-active biological product. O. Karpenko, N. Martinyuk, O.?Shulga, T. Pokynbroda, R. Vіldanova, N. Shcheglova. Appl. 25.12.2003; Publ. 12. 2004, Bul. № 12. (In Russian).
8. Karpenko E. V., Vildanova R. I., Shcheglova N. S. The prospect of using bacteria of genus Rhodococcus and microbial surfactants for degradation of oil pollutants. Appl. Biochem. Microbiol. 2006, 42 (2), 156–159.
https://doi.org/10.1134/S0003683806020074
9. Pat. Ukrainy. N 77228. МПК (2013.01), А01Р 21/00, А01N 63/00, С12N 1/02, С12R 1/38. The drug of complex action for use in agriculture and reclamation of technogenic modified soils. O. Karpenko, N. Shcheglova, R. Vіldanova, A. Shulga, V. Baranov. Applp. 13.06.2012; Publ. 11.02.2013, Bul. № 3. (In Ukrainian).
10. Wiesman Z., Ahronovitz A., Ronen A. Foliar fertilization with Olive Nutrivant for increase of olives and olive oil yields. Alon Hanotea. 2002, V. 56, Р. 128–132.
11. Karpenko E. V., Pokinbroda T. Ya., Makitra R. G., Palchikova E. Ya. Best practices highlight biogenic surfactants ramnolipidov. Zh. obshch. himii. 2009, N 12, 2011 p. (In Russian).
12. DSTU 4138-2002. Seeds crops. Methods for determining quality. Effective as of 1/1/2004. Кyiv: Derzhspozhyvstandart Ukrainy. 2003, 173 p. (In Ukrainian).
13. Segi Y. Methods of Soil Microbiology (Translation from Hungarian). Мoskva: Коlоs. 1983, 271 p.
14. Dospekhov B. Methods of field experience. Мoskva: Аgrоprоmizdat. 1985, 351 p. (In Russian).
15. Кyеfyеli V. I., Тuryetskaya R. Kh., Коf Е. М., Vlasov P. V. Methods for determination of phytohormones, growth inhibitors, defoliants and herbicides. Мoskva: Kоlоs. 1973, P. 7. (In Russian).
16. Lаkin А. N. Rate variation statistics. Кyiv: Vyshcha shkola. 1990, 116 p. (In Russian).
17. Akbar N. Barley grain for ruminants: A global treasure or tragedy. J. Anim. Sci. Biotechnol. 2012, 3 (22),1–9.
18. D’aes J., De Maeyer K. Pauwelyn D. Biosurfactants in plant – Pseudomonas interactions and their importance to biocontrol. Еnviron. Мicrobiol. Rep. 2010, 2 (3), 359–372.
19. Bunster L., Nyckle J., Schippers F. Effect of Surface-Active Pseudomonas spp. on Leaf Wettability. Appl. Environ. Microbiol. 1989, 55 (6), 1340–1345.
20. Раt.WO/1996/012685 US European Patent Office (EPO), PCT/EP1995/004004. Use of phospholipids to improve plant growth. Henkel C., Kopp-Holtwiesche A., Schlue W. Publication date 02.05.1996.
21. Sandstrom R. P., Cleland R. E. Selective Delipidation of the Plasma Membrane by Surfactants Enrichment of Sterols and Activation of ATPase. Plant Physiol. 1989, 90 (4), 1524–1531.
https://doi.org/10.1104/pp.90.4.1524
22. Shumilina E. V., Zonova N. Yu., Shchipunov Yu. A. Influence of surfactants on the activity of phospholipase DII. Biol. membr. 1998, 15 (4), 414–419. (In Russian).
23. Shcheglova N. S., Karpenko E. V., Vildanova?R. I. Effect of biogenic surfactant on growth of leguminous plants. Mater. Mezhd. konf. «Radostim», 24–25 November 2010, Krasnodar. P. 47–48. (In Russian).