ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta
V. 6, No 3, 2013
"Biotechnologia Acta" v. 6, no. 3, 2013
https://doi.org/10.15407/biotech6.03.110
Р. 110-115, Bibliography 18, Ukrainian.
Universal Decimal classification: 547.953:615.012:665.372
CREATING AND INVESTIGATION OF ADENOSINE-5-TRIPHOSPHATE
LIPOSOMAL FORMS FOR ORAL APPLYING
O. V. Khrobatenko, N. V. Prytulska, S. M. Shulga
Kyiv National University of Trade and Economics, Ukraine
State Organization «Institute of Food Biotechnology and Genomics»
of National Academy of Sciences of Ukraine, Kyiv
Liposomal form of adenosine-5-triphosphate (ATP) for oral applying was studied. The methods of obtaining and determining the size of the liposomes, the definition of ATP percentage content in liposomes were proposed. The effect of liposomal form of ATP on physical performance of laboratory animals by swimming to the limit of exhaustion was studied. It was shown that using of the liposomal form as compared with free ATP (in a similar dose) promoted the increased efficiency of laboratory animals. Application of liposomal form occurring at single administration for 6 days at a dose of 30 mg/kg facilated a 62% increase of efficiency, and 60 mg/kg did 76%, while consumption of liposomal form of ATP for 12 days did 64% and 93%, respectively.
Key words: liposomes, liposomal form ATP, physical performance.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013
References
1. Gordon J. L. Extracellular ATP: effects, sources and fate. Biochem. J. 1986, P. 309–319.
2. Spraiet L. Anaerobic metabolism during high-intensity physical activity. Metabolism during physical activity. Ed. M. Khargrivs. K.: Olimp. lit. 1998, P. 9–51. (In Russian).
3. Grin G. Metabolic fatigue factors. Metabolism during physical activity. Ed. M. Khargrivs. K.: Olimp. lit. 1998, P. 233–286. (In Russian).
4. Liang W., Levchenko T. S., Torchilin V. P. Encapsulation of ATP into liposomes by different methods: optimization of the procedure. J. Microencapsul. 2004, P.?251–261.
5. Hartner W. C., Verma D. D., Levchenko T. S. et al. ATP-loaded liposomes for treatment of myocardial ischemia. WIREs. Nanobiotechnol. 2009, P. 530–539.
6. Verma D. D., Levchenko T. S., Bernstein E. A., Torchilin V. P. ATP-loaded Liposomes Effectively Protect Mechanical Functions of the Myocardium from Global Ischemia in an Isolated Rat Heart Model. J. Control. Release. 2005, Р. 460–471.
7. Arakawa A., Ishiguro S., Ohki K., Tamai M. Preporation of liposome-encapsulationg adenosine triphosphate. Tohoku J. Exp. Med. 998, P. 39–47.
8. Morozova Yu. A. The liposomal delivery system of antigen EDS-76 for oral immunization of birds: Author. diss. ... Cand. biol. sciences:03.00.23. SPb. gos. tekhnoloh. in-t. SPb., 2003, 20 p. (In Russian).
9. Han Y. Y., Huang L., Jackson E. K. et al. Liposomal ATP or NAD+ protects human endothelial cells from energy failure in a cell culture model of sepsis. Res. Commun. Mol. Pathol. Pharmacol. 2001, P. 107–116.
10. Laham A., Claperon N., Durussel J. J. et al. Intracarotidal administration of liposomally-entrapped ATP: improved ef?ciency against experimental brain ischemia. Pharmacol. Res. Commun. 1988, P.?699–705.
11. Pat. 77865 UA, A23J 7/00, A61K 9/127, A61K 31/7076. Method of adenosine-5-triphosphate (ATP) obtaining of liposome form for oral use. Pritulska N. V., Khrobatenlko O. V., Shulga S. N. Appl. 20.09.2012; Publ. 25.02.13; Bul. N 4., 4 p.
12. The use of zeta potential measurements to study sterically stabilized liposomes [Electronic resource] — www.malvern.co.uk.
13. Scientific and practical advice on keeping laboratory animals and work with them. K.: Avitsena. 2002, 156?p. (In Ukrainian).
14. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe, Strasbourg, 1986. 53 p.
15. Moroz V. M., Lipnitskiy T. N., Kutniak V. P. The study and comparative evaluation of actoprotective activity ATFLONG in an experiment. Lik. sprava. 2002, N 7, P. 99–101. (In Russian).
16. Vdovenko N. V. Effect of adenozyntryfosfat compounds on prooxidant-antioxidant balance in conditions of intense exercise: Author. thesis. ... candidate. biol. sciences: 03.00.04. Nats. agrar. un-t. K. 2005, 20 p. (In Ukrainian).
17. Bobkov Yu. G., Vinogradov V. N. Pharmacological correction of fatigue. M.: Meditsina. 1984, 206 p. (In Russian).
18. Gubler E. V., Genkin A. A. Application of nonparametric statistics in biomedical research. L: Meditsina. 1973, 144 p. (In Russian).