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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" v. 6, no. 2, 2013
Р. 121-127, Bibliography 11, Russian.
Universal Decimal classification: 635.076:57.043


 L. V. Gorbunov1, I. V. Petrov2

1Institute of Animal Breeding of National Academy of Agricultural Sciences, Kharkіv
2Research Institute of Biology Karazin Kharkіv National University

Optimal cryopreservation parameters were determined for the berry-like crop cuttings: blackcurrant (Dachnica, Yuviley, Sofiivska, Kitayskaya); redcurrant (Kitaivska, Djoker, Svyatkova); gooseberries (Krasen, Malachite, Kolobok); raspberries (Novost’ Kuz’mina, Struyka, Skromnica). This procedure allowed increasing viability of deconservation samples in 2–5 times compared to the existing methods of cryopreservation. Maximal values of cutting viability were obtained after their temperature adaptation at –10 °C during 14–60 days, stepwise cooling of the samples with 0.1–0.5 °C/hr rate down to –30 °C (exposure 3–7 days), direct plunging into liquid nitrogen, storage from 1 to 30 days and thawing rate of 70 °C/min. It was found that the in the region of allowable values for maximum viability of investigated birch (control) cuttings humidity values correspond 32±40%, for blackcurrant are 40±50%, and raspberries 37±40 % in affirmative temperature span and 14±28 %, 30±40 %, 20±23 % in below zero.

Using dispersion analysis it has been established that the development probability of frozenthawed plant cuttings significantly depends on the selected cultivar and cryopreservation method. It is noted that the force of influence η2А, due to the individual properties of the studied cultivars of berries, is comparable to that of the force of influence ?2В of cryopreservation procedures.

The differences of average indexes of viability of the studied cultivars were showed within a single species. It was found that these rates are different for black currant by 92%, redcurrant — 54%, gooseberries — 48%, raspberries — 70%. Using paired Student’s t-test has reduced to 3 times the error of representativeness of the sample that enabled to improve the reliability of the significance of differences compared to the values of the P ≥0,99. Selection of the cuttings’ cultivars and cryopreservation method significantly affect the reproducibility of experimental results. The analysis of reproducibility of indexes of viability of the studied cuttings held by the coefficient of variation, showed that during the cryopreservation this value is changed to blackcurrant cultivars from 5 to 91%, redcurrant — 9–137%, gooseberries, 22–74%, raspberry 18–105%.

Key words: cuttings black and red currants, gooseberries, raspberries, viability, cryopreservation.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008