ISSN 1995-5537
«Біотехнологія» journal V. 5, No. 5, 2012
Р. 27-33, Bibliography 29, Ukrainian.
Universal Decimal classification: 575.827:604.6:582.683.2
FATTY ACID OIL COMPOSITION OF CANOLA CARRYING CYTOCHROME P450SCC CYP11A1 TRANSGENE
O. Sakhno, A. M. Ostapchuk, V. V. Klochko, M. V. Kuchuk
Institute of Cell Biology and Genetic Engineering of National Academy of Sciences of Ukraine, Kyiv
Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv
Using gas chromatography-mass spectrometry, qualitative and quantitative composition of fatty acids from transgenic rape Brassica napus L. var. oleifera DC. seeds was analyzed. The plants carrying bovine (Bos primigenius taurus L.) cytochrome P450SCC cyp11A1 gene due to agrobacterium transformation were grown under greenhouse conditions. It was shown that heterologous gene expression under constitutive (35S) promoter did not affect on total fatty acid content in seeds but resulted in quantitative changes in their composition. Second transformant generation was characterized by increasing of quantity of oleic acid on 6% (maximum up to 72.67 mol%) and decreasing of linolenic acid almost in half (up to 3.89 mol%). Our experiments give the possibility to describe more completely the obtained transgenic canola lines, which previously demonstrated increasing of total soluble protein in the leaves and seeds, increasing of antioxidant activity of leaf tissue, increasing activity of the superoxide dismutase during germination of seeds, decreasing of the period of vegetative development by 5–7 days and resistance for herbicide phosphinotricin treatment.
Key words: Brassica napus, cyp11A1, gas chromatography, fatty acids, rape, cytochrome P450SCC.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008