ISSN 1995-5537
"Biotechnology" journal V. 5, No. 2, 2012
Р. 86-91, Bibliography 16, Ukrainian.
Universal Decimal classification: 577.152.321+663.11
Donetsk National University
To increase endoglucanase (EC 3.2.1.4) production, selection of the cultivation conditions of basidiomycetes — active cellulolytic enzymes producers — was conducted along nutrient medium initial acidity (factor was changed between 3 and 9 pH with 1 pH step) and cultivation temperature (factor was changed between 24 and 36 °С with a step of 2 °С). Endoglucanase activity towards Na-carboxymethylcellulose was determined in the selection process. It was determined that optimal initial acidity of the nutrient medium for all strains was 7 pH, and cultivation temperature was 24 °С for the strains А-Дон-02 Irpex lacteus, AnSc-1 Daedaleopsis confragosa f. confragosa, Sh-1 Stereum hirsutum, 34 °С for the strain К-1 Irpex lacteus and 36 °С for the strain Д-1 Irpex lacteus. As the result of optimization, the values of endoglucanase activity increased at 1,59 times for the strain Sh-1 Stereum hirsutum, at 2,51 times for the strain А-Дон-02 Irpex lacteus, at 3,02 times for the strain К-1 Irpex lacteus, at 3,69 times for the the strain AnSc-1 Daedaleopsis confragosa f. confragosa and at 12,16 times for the strain Д-1 Irpex lacteus. At the same time, as the result of selection of cultivation conditions, specific endoglucanase activity of strain К-1 Irpex lacteus was increased at 3,54 times, А-Дон-02 Irpex lacteus — at 5,12 times, Д-1 Irpex lacteus—at 7,86 times and AnSc-1 Daedaleopsis confragosa f. confragosa — at 17,98 times. Culture J-2An Phellinus pomaceus shown absence of endoglucanase activity in any experiment. Maximal endoglucanase activity of enzymes in cultural liquids of strains К-1, А-Дон-02, Д-1 Irpex lacteus and AnSc-1 D. confragosa f. confragosa was established on the 7th day of cultivation, and for strain Sh-1 S. hirsutum — on the 14th day of the experiment.
Key words: basidiomycetes, endoglucanase, Na-carboxymethylcellulose, cultivation temperature, acidity of nutrient medium, optimization, Irpex lacteus, Stereum hirsutum, Daedaleopsis confragosa f. confragosa, Phellinus pomaceus.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008